CoraLite® Plus 488-conjugated NEU2 Recombinant antibody

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  IF Staining of PC-12 using CL488-83934-2

  Immunofluorescent analysis of (4% PFA) fixed PC-12 cells using CoraLite® Plus 488 NEU2 antibody (CL488-83934-2, Clone: 240685C12 ) at dilution of 1:200, CL594-Phalloidin (red).

NEU2 Recombinant Antibody for IF/ICC

Cat No. CL488-83934-2

Clone No.240685C12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Synonyms

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Tested Applications

• Positive IF/ICC detected in: PC-12 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-83934-2 targets NEU2 in IF/ICC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: NEU2 fusion protein Ag17386
• Full Name: sialidase 2 (cytosolic sialidase)
• Calculated Molecular Weight: 380 aa, 42 kDa
• GenBank Accession Number: BC107053
• Gene Symbol: NEU2
• Gene ID (NCBI): 4759
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9Y3R4
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

NEU2, also named as Sialidase 2, neuraminidase 2, catalyzes the hydrolytic cleavage of the terminal sialic acid (N-acetylneuraminic acid, Neu5Ac) of a glycan moiety in the catabolism of glycolipids, glycoproteins and oligosaccharides. NEU2 is localized in the cytosol and plasma membrane and takes part in myoblast and neuronal differentiation. It is almost undetectable under normal physiological conditions, and was found to be involved into apoptotic events in pancreatic ductal adenocarcinoma, one of the most dangerous cancers due to poor diagnosis and prognosis. (PMID: 30439363)