NEDD1 Recombinant antibody

• 

  WB analysis using 83443-6-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83443-6-RR (NEDD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  FC experiment of HEK-293 using 83443-6-RR

  1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug Nedd1 Recombinant Antibody (83443-6-RR, Clone:240404A7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

• 

  FC experiment of A431 using 83443-6-RR

  1x10^6 A431 cells were intracellularly stained with 0.25 ug Nedd1 Recombinant Antibody (83443-6-RR, Clone:240404A7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

• 

  Affinity and Kinetic Characterization of 83443-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83443-6-RR against Human NEDD1 were performed. The affinity constant is below 1 pM.

NEDD1 Uni-rAb^TM Recombinant Antibody for WB, FC (Intra), ELISA

Cat No. 83443-6-RR

Clone No.240404A7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Synonyms

Protein NEDD1, Neural precursor cell expressed developmentally down-regulated protein 1, NEDD-1, NEDD 1, 240404A7

---

Tested Applications

• Positive WB detected in: SH-SY5Y cells, HEK-293 cells, HeLa cells, Jurkat cells, MCF-7 cells
• Positive FC (Intra) detected in: A431 cells, HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83443-6-RR targets NEDD1 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: NEDD1 fusion protein Ag33603
• Full Name: neural precursor cell expressed, developmentally down-regulated 1
• Calculated Molecular Weight: 72 kDa
• Observed Molecular Weight: 71-75 kDa
• GenBank Accession Number: BC027605
• Gene Symbol: NEDD1
• Gene ID (NCBI): 121441
• RRID: AB_3671084
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q8NHV4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NEDD1 (NEDD1 gamma-tubulin ring complex targeting factor), also known as GCP-WD. It is expected to be located in centrosome; nucleoplasm; and plasma membrane. Required for mitosis progression. Promotes the nucleation of microtubules from the spindle. The molecular weight of NEDD1is 72 kDa.