NEDD1 Recombinant antibody, PBS Only

NEDD1 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240404A7

Cat no : 83443-6-PBS

Synonyms

Protein NEDD1, Neural precursor cell expressed developmentally down-regulated protein 1, NEDD-1, NEDD 1, 240404A7

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Validation Data Gallery

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  WB analysis using 83443-6-RR (same clone as 83443-6-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83443-6-RR (NEDD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83443-6-PBS in a different storage buffer formulation.

• 

  FC experiment of HEK-293 using 83443-6-RR (same clone as 83443-6-PBS)

  1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug Nedd1 Recombinant Antibody (83443-6-RR, Clone:240404A7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83443-6-PBS in a different storage buffer formulation.

• 

  FC experiment of A431 using 83443-6-RR (same clone as 83443-6-PBS)

  1x10^6 A431 cells were intracellularly stained with 0.25 ug Nedd1 Recombinant Antibody (83443-6-RR, Clone:240404A7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83443-6-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83443-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83443-6-RR against Human NEDD1 were performed. The affinity constant is below 1 pM.

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Product Information

The immunogen of 83443-6-PBS is NEDD1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: NEDD1 fusion protein Ag33603
• Full Name: neural precursor cell expressed, developmentally down-regulated 1
• Calculated Molecular Weight: 72 kDa
• GenBank Accession Number: BC027605
• Gene Symbol: NEDD1
• Gene ID (NCBI): 121441
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.