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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated NDP52 Monoclonal antibody

NDP52 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2F2C2

Cat no : CL594-66401

Synonyms

CALCOCO2, Nuclear dot protein 52, Nuclear domain 10 protein 52, Calcium-binding and coiled-coil domain-containing protein 2, 2F2C2

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using CoraLite®594-conjugated NDP52 Monoclonal antibody (CL594-66401)

    IF Staining of Jurkat using CL594-66401

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CoraLite®594 NDP52 antibody (CL594-66401, Clone: 2F2C2 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of Jurkat cells using CoraLite®594-conjugated NDP52 Monoclonal antibody (CL594-66401)

    FC experiment of Jurkat using CL594-66401

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human NDP52 (CL594-66401, Clone:2F2C2) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL594-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF/ICC detected inJurkat cells
Positive FC (Intra) detected inJurkat cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66401 targets NDP52 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen NDP52 fusion protein Ag2866
Full Name calcium binding and coiled-coil domain 2
Calculated Molecular Weight 446 aa, 52 kDa
Observed Molecular Weight 52 kDa
GenBank Accession NumberBC015893
Gene Symbol NDP52
Gene ID (NCBI) 10241
RRIDAB_2919986
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NDP52, also named as CALCOCO2, is an autophagy receptor. It plays a role in ruffle formation and actin cytoskeleton organization. Mouse/Rat NDP52 has some isoforms with MW 28-40 kDa and 67 kDa. Human NDP52 has some isoforms with MW 43-47 kDa and 52-55 kDa,

Protocols

Product Specific Protocols
IF protocol for CL594 NDP52 antibody CL594-66401Download protocol
Standard Protocols
Click here to view our Standard Protocols