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CoraLite® Plus 488-conjugated NDP52 Monoclonal antibody

NDP52 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2F2C2

Cat no : CL488-66401

Synonyms

CALCOCO2, NDP52, Nuclear domain 10 protein 52, Nuclear dot protein 52

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using CoraLite® Plus 488-conjugated NDP52 Monoclonal ant (CL488-66401)

    IF Staining of Jurkat using CL488-66401

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CoraLite® Plus 488 NDP52 antibody (CL488-66401, Clone: 2F2C2 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using CoraLite® Plus 488-conjugated NDP52 Monoclonal ant (CL488-66401)

    IF Staining of Jurkat using CL488-66401

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CoraLite® Plus 488 NDP52 antibody (CL488-66401, Clone: 2F2C2 ) at dilution of 1:200.

Tested Applications

Positive IF/ICC detected inJurkat cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66401 targets NDP52 in IF/ICC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen NDP52 fusion protein Ag2866
Full Name calcium binding and coiled-coil domain 2
Calculated Molecular Weight 446 aa, 52 kDa
Observed Molecular Weight 52 kDa
GenBank Accession NumberBC015893
Gene Symbol NDP52
Gene ID (NCBI) 10241
RRIDAB_2919321
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NDP52, also named as CALCOCO2, is an autophagy receptor. It plays a role in ruffle formation and actin cytoskeleton organization. Mouse/Rat NDP52 has some isoforms with MW 28-40 kDa and 67 kDa. Human NDP52 has some isoforms with MW 43-47 kDa and 52-55 kDa,

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 NDP52 antibody CL488-66401Download protocol
Standard Protocols
Click here to view our Standard Protocols