CoraLite®594-conjugated NDE1 Polyclonal antibody

• Featured Product
• KD/KO Validated

NDE1 Polyclonal Antibody for IF-P

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF-P

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-10233

Synonyms

FLJ20101, HOM TES 87, NDE1, NUDE, NUDE1

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Validation Data Gallery

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  IF Staining of human breast cancer using CL594-10233

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite®594 NDE1 antibody (CL594-10233) at dilution of 1:200.

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  IF Staining of human breast cancer using CL594-10233

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite®594 NDE1 antibody (CL594-10233) at dilution of 1:200.

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Tested Applications

• Positive IF-P detected in: human breast cancer tissue

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-10233 targets NDE1 in IF-P applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NDE1 fusion protein Ag0336
• Full Name: nudE nuclear distribution gene E homolog 1 (A. nidulans)
• Calculated Molecular Weight: 335 aa, 38 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC001421
• Gene Symbol: NDE1
• Gene ID (NCBI): 54820
• RRID: AB_2919725
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage.

Background Information

NDE1 (NUDE /NUDE1) is a LIS1-interacting protein. A signaling pathway containing NDE1, LIS1, and cytoplasmic dynein is conserved for eukaryotic nuclear migration and has profound impact on neuronal migration during development of the central nervous system. LIS1 and dynein have been shown to be involved in mitosis and NDE1 participates in a subset of dynein function in M phase. NDE1 is specifically phosphorylated in M phase in its serine/threonine phosphorylation motifs, probably by Cdc2 and also Erk1 and Erk2. A fraction of NDEl binds to centrosomes strongly in interphase and is localized to mitotic spindles in early M phase. Phosphorylation of NDE1 also regulates the cell-cycle-dependent distribution, possibly by increasing its dissociation rate at the microtubule-organizing center. This antibody is a rabbit polyclonal antibody raised against a peptide mapping within human NDE1.