NDE1 Polyclonal antibody

• Featured Product
• KD/KO Validated

NDE1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

37

Reviews (2)

Cat no : 10233-1-AP

Synonyms

NUDE1, NudE, HOM TES 87

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Validation Data Gallery

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  WB analysis using 10233-1-AP

  Various cells were subjected to SDS PAGE followed by western blot with 10233-1-AP (NDE1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A2780 using 10233-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 10233-1-AP (NDE1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10233-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10233-1-AP (NDE1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10233-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10233-1-AP (NDE1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 10233-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 10233-1-AP (NDE1 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 10233-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10233-1-AP (NDE1 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 10233-1-AP

  IP result of anti-NDE1 (IP:10233-1-AP, 3ug; Detection:10233-1-AP 1:1500) with HEK-293 cells lysate 4500ug.

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  IHC staining of human breast cancer using 10233-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10233-1-AP (NDE1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human breast cancer using 10233-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10233-1-AP (NDE1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human colon cancer using 10233-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10233-1-AP (NDE1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human colon cancer using 10233-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10233-1-AP (NDE1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of human breast cancer using 10233-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using NDE1 antibody (10233-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human breast cancer using 10233-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using NDE1 antibody (10233-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of MCF-7 using 10233-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using NDE1 antibody (10233-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

• Positive WB detected in: A2780 cells, HEK-293 cells, HeLa cells, rat liver tissue, mouse liver tissue, HepG2 cells, mouse brain tissue, rat brain tissue
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human breast cancer tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human breast cancer tissue
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 9 publications below
• WB: See 25 publications below
• IHC: See 2 publications below
• IF: See 20 publications below
• IP: See 1 publications below
• CoIP: See 2 publications below

Product Information

10233-1-AP targets NDE1 in WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NDE1 fusion protein Ag0336
• Full Name: nudE nuclear distribution gene E homolog 1 (A. nidulans)
• Calculated Molecular Weight: 335 aa, 38 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC001421
• Gene Symbol: NDE1
• Gene ID (NCBI): 54820
• RRID: AB_2149877
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NDE1 (NUDE /NUDE1) is a LIS1-interacting protein. A signaling pathway containing NDE1, LIS1, and cytoplasmic dynein is conserved for eukaryotic nuclear migration and has profound impact on neuronal migration during development of the central nervous system. LIS1 and dynein have been shown to be involved in mitosis and NDE1 participates in a subset of dynein function in M phase. NDE1 is specifically phosphorylated in M phase in its serine/threonine phosphorylation motifs, probably by Cdc2 and also Erk1 and Erk2. A fraction of NDEl binds to centrosomes strongly in interphase and is localized to mitotic spindles in early M phase. Phosphorylation of NDE1 also regulates the cell-cycle-dependent distribution, possibly by increasing its dissociation rate at the microtubule-organizing center. This antibody is a rabbit polyclonal antibody raised against a peptide mapping within human NDE1.

Publications

Species	Application	Title
mouse	IF	Science; A liquid-like spindle domain promotes acentrosomal spindle assembly in mammalian oocytes.; Authors - Chun So
human,mouse	WB,IF	Nat Cell Biol; Nde1-mediated inhibition of ciliogenesis affects cell cycle re-entry.; Authors - Kim Sehyun S ; KD Validated
human	WB	Neuron; Posterior Neocortex-Specific Regulation of Neuronal Migration by CEP85L Identifies Maternal Centriole-Dependent Activation of CDK5.; Authors - Andrew Kodani
human,mouse	WB,IHC,IF	Am J Hum Genet; The essential role of centrosomal NDE1 in human cerebral cortex neurogenesis.; Authors - Bakircioglu Mehmet M
human	WB	J Cell Biol; Mitotic control of kinetochore-associated dynein and spindle orientation by human Spindly.; Authors - Chan Ying Wai YW
human	WB,IF	EMBO J; CPAP promotes timely cilium disassembly to maintain neural progenitor pool.; Authors - Elke Gabriel

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Philip (Verified Customer) (09-04-2019)

Cells fixed in PTEMF for 10min at RT. Blocked in PBS+3% BSA for 30min at RT. Primary at 1/500 for 1hr at RT.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: HeLa K

FH

Aurelie (Verified Customer) (09-01-2019)

lysates (15µg) from human primary Fibroblasts were loaded in 8% SDS-PAGE and immunoblotted using anti-Nde1 ( 1/1500) human primary Fibroblasts were fixed in PFA 4% and stained using anti-Nde1 ( 1/100) over-night at 4°C followed by 1h at RT with a secondary anti-rabbit Alexa (594nM) .

• Applications: Western Blot, Immunofluorescence,
• Primary Antibody Dilution: 1/1500 (WB) et 1/100 (IF)
• Cell Tissue Type: primary human fibroblasts

NDE1 Antibody Western Blot,Immunofluorescence, validation (1/1500 (WB) et 1/100 (IF) dilution) in primary human fibroblasts (Cat no:10233-1-AP)

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