FcZero-rAb™ PE Anti-Human N-cadherin/CD325 Rabbit Recombinant Antibody

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  FC experiment of HeLa using PE-FcA98006

  1x10^6 HeLa cells (left) or MCF-7 cells (right) were surface stained with 5 ul PE Anti-Human N-cadherin/CD325 Rabbit RecAb (PE-FcA98006, Clone: 230259D7) (red), or 5 ul PE Rabbit IgG Isotype Control RecAb (PE-FcA98136, Clone: 240953C9) (blue). Cells were not fixed.

N-cadherin/CD325 FcZero-rAb^TM Recombinant Antibody for FC

Cat No. PE-FcA98006

Clone No.230259D7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

N-cadherin, CDHN, CDH2, CD325, Cadherin-2

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Tested Applications

• Positive FC detected in: HeLa cells

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is N/A per 10^6 cells in a 100 µl suspension or N/A per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

PE-FcA98006 targets N-cadherin/CD325 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: cadherin 2, type 1, N-cadherin (neuronal)
• Calculated Molecular Weight: 100 kDa
• GenBank Accession Number: NM_001792.4
• Gene Symbol: N-cadherin
• Gene ID (NCBI): 1000
• Conjugate: PE Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. N-cadherin (neural cadherin), also known as CDH2 (cadherin 2), is a 130-kDa transmembrane protein and a classical member of the cadherin superfamily which also include E-, P-, R-, and B-cadherins. Expression of N-cadherin has been reported on various cell types including neurons, endothelial cells and cardiac myocytes (PMID: 11282032; 9508779; 8125202). N-cadherin has functions in early brain morphogenesis, synaptogenesis and synaptic plasticity (PMID: 23321619).