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Recombinant Human N-Cadherin protein (His Tag)

Species

Human

Purity

>90 %, SDS-PAGE

Tag

His Tag

Activity

not tested

Cat no : Eg0701

Validation Data Gallery

  • Purity of Recombinant Human N-Cadherin was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Human N-Cadherin was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

Product Information

Purity >90 %, SDS-PAGE
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Human N-Cadherin protein Asp160-Ala724 (Accession# P19022-1) with a His tag at the C-terminus.
GeneID 1000
Accession P19022-1
PredictedSize 62.9 kDa
SDS-PAGE 70-90 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. N-cadherin (neural cadherin), also known as CDH2 (cadherin 2), is a 130-kDa transmembrane protein and a classical member of the cadherin superfamily which also include E-, P-, R-, and B-cadherins. Expression of N-cadherin has been reported on various cell types including neurons, endothelial cells and cardiac myocytes. N-cadherin has functions in early brain morphogenesis, synaptogenesis and synaptic plasticity. Soluble N-cadherin (sN-CAD) is found in biological fluids, including serum, seminal fluid samples, and urine. It has been shown that significantly higher amounts of serum sN-CAD are present in cancer patients than in persons with no evidence of disease.

References:

1. Navarro, P et al. (1998) J Cell Biol.140(6):1475-1484. 
2. Derycke, Lara et al. (2006) Int J Cancer.119(12):2895-2900. 
3. Moya, Pablo R et al. (2013) Eur J Hum Genet.21(8):850-854.