CoraLite® Plus 488-conjugated N-PAC Monoclonal antibody

N-PAC Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1A5E1

Cat no : CL488-67340

Synonyms

GLYR1, HIBDL, Glyoxylate reductase 1 homolog, Cytokine-like nuclear factor N-PAC, 3-hydroxyisobutyrate dehydrogenase-like protein

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Validation Data Gallery

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  IF Staining of HeLa using CL488-67340

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 N-PAC antibody (CL488-67340, Clone: 1A5E1 ) at dilution of 1:600.

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  FC experiment of HeLa using CL488-67340

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human N-PAC (CL488-67340, Clone:1A5E1) and at dilution 1:0 (red), or 0.4 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:300-1:1200
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67340 targets N-PAC in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: N-PAC fusion protein Ag7133
• Full Name: cytokine-like nuclear factor n-pac
• Calculated Molecular Weight: 61 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC064940
• Gene Symbol: N-PAC
• Gene ID (NCBI): 84656
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.