Filter: AllWBIHCIF/ICC

WB analysis using 60229-1-Ig (same clone as 60229-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 60229-1-Ig (Myosin Light Chain 2/MLC-2V antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60229-1-PBS in a different storage buffer formulation.
WB analysis of pig heart using 60229-1-Ig (same clone as 60229-1-PBS)
pig heart tissue were subjected to SDS PAGE followed by western blot with 60229-1-Ig (Myosin Light Chain 2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60229-1-PBS in a different storage buffer formulation.
WB analysis of rat heart using 60229-1-Ig (same clone as 60229-1-PBS)
rat heart tissue were subjected to SDS PAGE followed by western blot with 60229-1-Ig (Myosin Light Chain 2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60229-1-PBS in a different storage buffer formulation.
IHC staining of human heart using 60229-1-Ig (same clone as 60229-1-PBS)
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 60229-1-Ig (Myosin Light Chain 2 antibody) at dilution of 1:500 (under 0x lens). This data was developed using the same antibody clone with 60229-1-PBS in a different storage buffer formulation.
IHC staining of human heart using 60229-1-Ig (same clone as 60229-1-PBS)
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 60229-1-Ig (Myosin Light Chain 2 antibody) at dilution of 1:500 (under 0x lens). This data was developed using the same antibody clone with 60229-1-PBS in a different storage buffer formulation.
IHC staining of human heart using 60229-1-Ig (same clone as 60229-1-PBS)
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 60229-1-Ig (Myosin Light Chain 2 antibody) at dilution of 1:500 (under 10x lens). This data was developed using the same antibody clone with 60229-1-PBS in a different storage buffer formulation.
IHC staining of mouse skeletal muscle using 60229-1-Ig (same clone as 60229-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 60229-1-Ig (Myosin Light Chain 2/MLC-2V antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60229-1-PBS in a different storage buffer formulation.
IHC staining of mouse heart using 60229-1-Ig (same clone as 60229-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 60229-1-Ig (Myosin Light Chain 2/MLC-2V antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60229-1-PBS in a different storage buffer formulation.
IF Staining of C2C12 using 60229-1-Ig (same clone as 60229-1-PBS)
Immunofluorescent analysis of (-20°C Methanol) fixed C2C12 cells using Myosin Light Chain 2/MLC-2V antibody (60229-1-Ig, Clone: 5A1F1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60229-1-PBS in a different storage buffer formulation.

Myosin Light Chain 2/MLC-2V Monoclonal antibody, PBS Only

Myosin Light Chain 2/MLC-2V Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 60229-1-PBS

Clone No.5A1F1

Review Product

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

MLC 2v, MYL2, MLC-2s/v, MLC-2, MLC2

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

60229-1-PBS targets Myosin Light Chain 2/MLC-2V in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Reactivity	human, mouse, rat, pig, rabbit
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	Myosin Light Chain 2/MLC-2V fusion protein Ag1356 Predict reactive species
Full Name	myosin, light chain 2, regulatory, cardiac, slow
Calculated Molecular Weight	19 kDa
Observed Molecular Weight	18 kDa
GenBank Accession Number	BC015821
Gene Symbol	Myosin Light Chain 2
Gene ID (NCBI)	4633
RRID	AB_2881356
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P10916
Storage Buffer	PBS only , pH 7.3
Storage Conditions	Store at -80°C.

Background Information

MYL2, also named as MLC-2v and MLC-2, is ventricular/cardiac muscle isoform. Defects in MYL2 are the cause of cardiomyopathy familial hypertrophic type 10 (CMH10). Defects in MYL2 are the cause of cardiomyopathy familial hypertrophic with mid-left ventricular chamber type 2 (MVC2). MYL2 has been widely used as a marker of mature ventricular cardiomyocytes.