Myosin Light Chain 2/MLC-2V Monoclonal antibody

Myosin Light Chain 2/MLC-2V Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

3

CloneNo.

5A1F1

Reviews (1)

Cat no : 60229-1-Ig

Synonyms

CMH10, MLC 2, MLC 2v, MLC2, MLC2v, MYL2, Myosin Light Chain 2, Myosin Light Chain 2/MLC-2V

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Validation Data Gallery

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  WB analysis using 60229-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60229-1-Ig (Myosin Light Chain 2/MLC-2V antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig heart using 60229-1-Ig

  pig heart tissue were subjected to SDS PAGE followed by western blot with 60229-1-Ig (Myosin Light Chain 2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat heart using 60229-1-Ig

  rat heart tissue were subjected to SDS PAGE followed by western blot with 60229-1-Ig (Myosin Light Chain 2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human heart using 60229-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 60229-1-Ig (Myosin Light Chain 2 antibody) at dilution of 1:500 (under 0x lens).

• 

  IHC staining of human heart using 60229-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 60229-1-Ig (Myosin Light Chain 2 antibody) at dilution of 1:500 (under 0x lens).

• 

  IHC staining of mouse skeletal muscle using 60229-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 60229-1-Ig (Myosin Light Chain 2/MLC-2V antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 60229-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 60229-1-Ig (Myosin Light Chain 2/MLC-2V antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of C2C12 using 60229-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed C2C12 cell using 60229-1-Ig (Myosin Light Chain 2 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of C2C12 using 60229-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed C2C12 cells using Myosin Light Chain 2/MLC-2V antibody (60229-1-Ig, Clone: 5A1F1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: human heart tissue, pig heart tissue, rat heart tissue, rabbit heart tissue
• Positive IHC detected in: mouse heart tissue, mouse skeletal muscle tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: C2C12 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 2 publications below

Product Information

60229-1-Ig targets Myosin Light Chain 2/MLC-2V in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

• Tested Reactivity: human, mouse, rat, pig, rabbit
• Cited Reactivity: human, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Myosin Light Chain 2/MLC-2V fusion protein Ag1356
• Full Name: myosin, light chain 2, regulatory, cardiac, slow
• Calculated Molecular Weight: 19 kDa
• Observed Molecular Weight: 18 kDa
• GenBank Accession Number: BC015821
• Gene Symbol: Myosin Light Chain 2
• Gene ID (NCBI): 4633
• RRID: AB_2881356
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MYL2, also named as MLC-2v and MLC-2, is ventricular/cardiac muscle isoform. Defects in MYL2 are the cause of cardiomyopathy familial hypertrophic type 10 (CMH10). Defects in MYL2 are the cause of cardiomyopathy familial hypertrophic with mid-left ventricular chamber type 2 (MVC2). MYL2 has been widely used as a marker of mature ventricular cardiomyocytes.

Publications

Species	Application	Title
human	IF	Stem Cells; Nitric Oxide-cGMP-PKG Pathway Acts on Orai1 to Inhibit the Hypertrophy of Human Embryonic Stem Cell-Derived Cardiomyocytes.; Authors - Y Wang
rat	WB	Int J Stem Cells; Synergistic Effect of Hydrogen and 5-Aza on Myogenic Differentiation through the p38 MAPK Signaling Pathway in Adipose-Derived Mesenchymal Stem Cells; Authors - Jingcheng Wang
human	IF	Stem Cell Rev Rep; MYOCD is Required for Cardiomyocyte-like Cells Induction from Human Urine Cells and Fibroblasts Through Remodeling Chromatin.; Authors - Xiangyu Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Irem (Verified Customer) (04-19-2022)

Unfortunately, this antibody unspecifically binds to many proteins in Jurkat cells.

• Applications: Western Blot
• Primary Antibody Dilution: 1:800
• Cell Tissue Type: Jurkat cells

Myosin Light Chain 2/MLC-2V Antibody Western Blot validation (1:800 dilution) in Jurkat cells (Cat no:60229-1-Ig)