Myoglobin Monoclonal antibody, PBS Only

Myoglobin Monoclonal Antibody for Indirect ELISA, IHC, WB

Host / Isotype

Mouse / IgG1

Reactivity

rabbit, pig, rat, mouse, human

Applications

Indirect ELISA, IHC, WB

Conjugate

Unconjugated

CloneNo.

1A4F7

Cat no : 66205-1-PBS

Synonyms

MB, Pseudoperoxidase MB, Nitrite reductase MB, EC:1.7.-.-, EC:1.11.1.-

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Validation Data Gallery

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  WB analysis of human skeletal muscle using 66205-1-Ig (same clone as 66205-1-PBS)

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66205-1-Ig (Myoglobin antibody at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66205-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human heart using 66205-1-Ig (same clone as 66205-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 66205-1-Ig (Myoglobin antibody at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66205-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rabbit heart using 66205-1-Ig (same clone as 66205-1-PBS)

  rabbit heart tissue were subjected to SDS PAGE followed by western blot with 66205-1-Ig (Myoglobin antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66205-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig heart using 66205-1-Ig (same clone as 66205-1-PBS)

  pig heart tissue were subjected to SDS PAGE followed by western blot with 66205-1-Ig (Myoglobin antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66205-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat heart using 66205-1-Ig (same clone as 66205-1-PBS)

  rat heart tissue were subjected to SDS PAGE followed by western blot with 66205-1-Ig (Myoglobin antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66205-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse heart using 66205-1-Ig (same clone as 66205-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 66205-1-Ig (Myoglobin antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66205-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 66205-1-Ig (same clone as 66205-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66205-1-Ig (Myoglobin antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66205-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat heart using 66205-1-Ig (same clone as 66205-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 66205-1-Ig (Myoglobin antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66205-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse tongue using 66205-1-Ig (same clone as 66205-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse tongue tissue slide using 66205-1-Ig (Myoglobin antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66205-1-PBS in a different storage buffer formulation.

• 

  FC experiment of C2C12 using 66205-1-Ig (same clone as 66205-1-PBS)

  1X10^6 C2C12 cells were intracellularly stained with 0.4 ug Anti-Human Myoglobin (66205-1-Ig, Clone:1A4F7) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66205-1-PBS in a different storage buffer formulation.

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Product Information

66205-1-PBS targets Myoglobin in Indirect ELISA, IHC, WB applications and shows reactivity with rabbit, pig, rat, mouse, human samples.

• Tested Reactivity: rabbit, pig, rat, mouse, human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Myoglobin fusion protein Ag8979
• Full Name: myoglobin
• Calculated Molecular Weight: 154 aa, 17 kDa
• Observed Molecular Weight: 17-18 kDa
• GenBank Accession Number: BC014547
• Gene Symbol: Myoglobin
• Gene ID (NCBI): 4151
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Myoglobin is a cytoplasmic hemoprotein that is expressed primarily in cardiomyocytes and oxidative skeletal muscle fibers, functioning on facilitating oxygen transport and modulating nitric oxide homeostasis within cardiac and skeletal myocytes. Recent studies indicated that myoglobin was also expressed in non-muscle tissues. This antibody well recognized endogenous myoglobin in muscle lysates.