Myocilin Monoclonal antibody, PBS Only

Myocilin Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, pig, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4B1F6

Cat no : 60357-1-PBS

Synonyms

GLC1A, GPOA, JOAG, JOAG1, MYOC, myocilin, TIGR

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Validation Data Gallery

• 

  WB analysis of pig heart using 60357-1-Ig (same clone as 60357-1-PBS)

  pig heart tissue were subjected to SDS PAGE followed by western blot with 60357-1-Ig (Myocilin antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60357-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat heart using 60357-1-Ig (same clone as 60357-1-PBS)

  rat heart tissue were subjected to SDS PAGE followed by western blot with 60357-1-Ig (Myocilin antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60357-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human skeletal muscle using 60357-1-Ig (same clone as 60357-1-PBS)

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 60357-1-Ig (Myocilin antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60357-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human spleen using 60357-1-Ig (same clone as 60357-1-PBS)

  human spleen tissue were subjected to SDS PAGE followed by western blot with 60357-1-Ig (Myocilin antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60357-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 60357-1-Ig (same clone as 60357-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 60357-1-Ig (Myocilin antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60357-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 60357-1-Ig (same clone as 60357-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 60357-1-Ig (Myocilin antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60357-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skeletal muscle using 60357-1-Ig (same clone as 60357-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 60357-1-Ig (Myocilin antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60357-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skeletal muscle using 60357-1-Ig (same clone as 60357-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 60357-1-Ig (Myocilin antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60357-1-PBS in a different storage buffer formulation.

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Product Information

60357-1-PBS targets Myocilin in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, pig, rat samples.

• Tested Reactivity: human, mouse, pig, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: Myocilin fusion protein Ag5492
• Full Name: myocilin, trabecular meshwork inducible glucocorticoid response
• Calculated Molecular Weight: 57 kDa
• Observed Molecular Weight: 50-57 kDa
• GenBank Accession Number: BC029261
• Gene Symbol: Myocilin
• Gene ID (NCBI): 4653
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Myocilin (MYOC), also known as TIGR or GLC1A, is a secreted glycoprotein of the olfactomedin family, originally identified in trabecular meshwork cells after prolonged treatment with glucocorticoids, and, independently, in the retina (PMID: 9176893; 9169133). It may participate in the obstruction of fluid outflow in the trabecular meshwork. Defects in MYOC are the cause of primary open angle glaucoma (POAG) (PMID: 21709622).