Myocilin Monoclonal antibody

Myocilin Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, pig, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

4B1F6

Cat no : 60357-1-Ig

Synonyms

GLC1A, GPOA, JOAG, JOAG1, MYOC, myocilin, TIGR



Tested Applications

Positive WB detected inpig heart tissue, human skeletal muscle tissue, human spleen tissue, rat heart tissue
Positive IHC detected inhuman heart tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60357-1-Ig targets Myocilin in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, pig, rat samples.

Tested Reactivity human, mouse, pig, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Myocilin fusion protein Ag5492
Full Name myocilin, trabecular meshwork inducible glucocorticoid response
Calculated Molecular Weight 57 kDa
Observed Molecular Weight 50-57 kDa
GenBank Accession NumberBC029261
Gene Symbol Myocilin
Gene ID (NCBI) 4653
RRIDAB_2881465
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Myocilin (MYOC), also known as TIGR or GLC1A, is a secreted glycoprotein of the olfactomedin family, originally identified in trabecular meshwork cells after prolonged treatment with glucocorticoids, and, independently, in the retina (PMID: 9176893; 9169133). It may participate in the obstruction of fluid outflow in the trabecular meshwork. Defects in MYOC are the cause of primary open angle glaucoma (POAG) (PMID: 21709622).

Protocols

Product Specific Protocols
WB protocol for Myocilin antibody 60357-1-IgDownload protocol
IHC protocol for Myocilin antibody 60357-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
IHC

Stem Cell Res Ther

Human amniotic epithelial cell transplantation improves scar remodeling in a rabbit model of acute vocal fold injury: a pilot study.

Authors - Yourka D Tchoukalova
ratWB

Exp Eye Res

Sympathetic activation leads to Schlemm's canal expansion via increasing vasoactive intestinal polypeptide secretion from trabecular meshwork

Authors - Dingwen Xu
humanWB,IF

Int Ophthalmol

The effect of ET1-CTGF mediated pathway on the accumulation of extracellular matrix in the trabecular meshwork and its contribution to the increase in IOP

Authors - Junming Wang
humanWB

Transl Vis Sci Technol

Inhibition of TGF-β2-Induced Trabecular Meshwork Fibrosis by Pirfenidone

Authors - Xiaofeng Zhu
mouseIF

Res Sq

Lentiviral mediated delivery of CRISPR/Cas9 reduces intraocular pressure in a mouse model of myocilin glaucoma

Authors - Shruti V Patil
mouseIF

Sci Rep

Lentiviral mediated delivery of CRISPR/Cas9 reduces intraocular pressure in a mouse model of myocilin glaucoma

Authors - Shruti V Patil