Myocilin Monoclonal antibody
Myocilin Monoclonal Antibody for WB, IHC, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
human, mouse, pig, rat
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
CloneNo.
4B1F6
Cat no : 60357-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | pig heart tissue, human skeletal muscle tissue, human spleen tissue, rat heart tissue |
Positive IHC detected in | human heart tissue, human skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:8000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 6 publications below |
IHC | See 1 publications below |
IF | See 4 publications below |
Product Information
60357-1-Ig targets Myocilin in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, pig, rat samples.
Tested Reactivity | human, mouse, pig, rat |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Immunogen | Myocilin fusion protein Ag5492 |
Full Name | myocilin, trabecular meshwork inducible glucocorticoid response |
Calculated Molecular Weight | 57 kDa |
Observed Molecular Weight | 50-57 kDa |
GenBank Accession Number | BC029261 |
Gene Symbol | Myocilin |
Gene ID (NCBI) | 4653 |
RRID | AB_2881465 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Myocilin (MYOC), also known as TIGR or GLC1A, is a secreted glycoprotein of the olfactomedin family, originally identified in trabecular meshwork cells after prolonged treatment with glucocorticoids, and, independently, in the retina (PMID: 9176893; 9169133). It may participate in the obstruction of fluid outflow in the trabecular meshwork. Defects in MYOC are the cause of primary open angle glaucoma (POAG) (PMID: 21709622).
Protocols
Product Specific Protocols | |
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WB protocol for Myocilin antibody 60357-1-Ig | Download protocol |
IHC protocol for Myocilin antibody 60357-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Stem Cell Res Ther Human amniotic epithelial cell transplantation improves scar remodeling in a rabbit model of acute vocal fold injury: a pilot study. | ||
Exp Eye Res Sympathetic activation leads to Schlemm's canal expansion via increasing vasoactive intestinal polypeptide secretion from trabecular meshwork | ||
bioRxiv Ocular hypertension impairs axonal transport in the optic nerve head leading to neurodegeneration in a novel Cre-inducible mouse model of myocilin glaucoma | ||
Biochem Biophys Res Commun Identification and application of p75 neurotrophin receptor-expressing human trabecular meshwork progenitor cells. | ||
Int Ophthalmol The effect of ET1-CTGF mediated pathway on the accumulation of extracellular matrix in the trabecular meshwork and its contribution to the increase in IOP | ||
Transl Vis Sci Technol Inhibition of TGF-β2-Induced Trabecular Meshwork Fibrosis by Pirfenidone |