CoraLite®594-conjugated Mu Crystallin Polyclonal antibody

Mu Crystallin Polyclonal Antibody for IF-P, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF-P, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

Publications

1

Cat no : CL594-12495

Synonyms

CRYM, NADP-regulated thyroid-hormone-binding protein, Ketimine reductase mu-crystallin, EC:1.5.1.25, DFNA40

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Validation Data Gallery

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  IF Staining of human gliomas using CL594-12495

  Immunofluorescent analysis of (4% PFA) fixed human gliomas tissue using CoraLite®594 Mu Crystallin antibody (CL594-12495) at dilution of 1:200.

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  FC experiment of SH-SY5Y using CL594-12495

  1X10^6 SH-SY5Y cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human Mu Crystallin (CL594-12495) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF-P detected in: human gliomas tissue
• Positive FC (Intra) detected in: SH-SY5Y cells

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

CL594-12495 targets Mu Crystallin in IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Mu Crystallin fusion protein Ag3161
• Full Name: crystallin, mu
• Calculated Molecular Weight: 314 aa, 34 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC018061
• Gene Symbol: Mu Crystallin
• Gene ID (NCBI): 1428
• RRID: AB_2919784
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

mu Crystallin(thiomorpholine-carboxylate dehydrogenase) is also named as THBP, CRYM, ketimine reductase and belongs to the ornithine cyclodeaminase family. This protein catalyzes the reduction of imine bonds in brain substrates that may include cystathionine ketimine (CysK) and lanthionine ketimine (LK). It is also involved in the regulation of the free intracellular concentration of triiodothyronine and access to its nuclear receptor.

Publications

Species	Application	Title
	IF	Cell Stem Cell; SYF2 suppression mitigates neurodegeneration in models of diverse forms of ALS; Authors - Gabriel R Linares