Mu Crystallin Polyclonal antibody

Mu Crystallin Polyclonal Antibody for WB, IHC, IF-P, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, FC (Intra), ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 12495-1-AP

Synonyms

CRYM, NADP-regulated thyroid-hormone-binding protein, Ketimine reductase mu-crystallin, EC:1.5.1.25, DFNA40

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Validation Data Gallery

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  WB analysis of Y79 using 12495-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 12495-1-AP (mu Crystallin antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 12495-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 12495-1-AP (mu Crystallin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 12495-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12495-1-AP (mu Crystallin antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 12495-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 12495-1-AP (mu Crystallin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human gliomas using 12495-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12495-1-AP (mu Crystallin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 12495-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12495-1-AP (mu Crystallin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human gliomas using 12495-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human gliomas tissue using Mu Crystallin antibody (12495-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of human gliomas using 12495-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human gliomas tissue using Mu Crystallin antibody (12495-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  FC experiment of SH-SY5Y using 12495-1-AP

  1X10^6 SH-SY5Y cells were intracellularly stained with 0.2 ug Anti-Human Mu Crystallin (12495-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: Y79 cells, Jurkat cells, human kidney tissue, human heart tissue
• Positive IHC detected in: human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human gliomas tissue
• Positive FC (Intra) detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Product Information

12495-1-AP targets Mu Crystallin in WB, IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Mu Crystallin fusion protein Ag3161
• Full Name: crystallin, mu
• Calculated Molecular Weight: 314 aa, 34 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC018061
• Gene Symbol: Mu Crystallin
• Gene ID (NCBI): 1428
• RRID: AB_2084620
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

mu Crystallin(thiomorpholine-carboxylate dehydrogenase) is also named as THBP, CRYM, ketimine reductase and belongs to the ornithine cyclodeaminase family. This protein catalyzes the reduction of imine bonds in brain substrates that may include cystathionine ketimine (CysK) and lanthionine ketimine (LK). It is also involved in the regulation of the free intracellular concentration of triiodothyronine and access to its nuclear receptor.

Publications

Species	Application	Title
mouse	IF	Dev Cell; Single-Cell Transcriptomic Analyses of the Developing Meninges Reveal Meningeal Fibroblast Diversity and Function.; Authors - John DeSisto
rat	WB	Endocr J; Follicular thyroglobulin enhances gene expression necessary for thyroid hormone secretion.; Authors - Yuko Ishido
rat	WB,IHC,IF	Neuropathology; Expression of CRYM in different rat organs during development and its decreased expression in degenerating pyramidal tracts in amyotrophic lateral sclerosis.; Authors - Reiji Hommyo

Related Products

Flow Cytometry Perm Buffer (10X)

PF00011-C