Y79 cells were subjected to SDS PAGE followed by western blot with 12495-1-AP (mu Crystallin antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
Y79 cells were subjected to SDS PAGE followed by western blot with 12495-1-AP (mu Crystallin antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of human kidney using 12495-1-AP
human kidney tissue were subjected to SDS PAGE followed by western blot with 12495-1-AP (mu Crystallin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
human kidney tissue were subjected to SDS PAGE followed by western blot with 12495-1-AP (mu Crystallin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 12495-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 12495-1-AP (mu Crystallin antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
Jurkat cells were subjected to SDS PAGE followed by western blot with 12495-1-AP (mu Crystallin antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of human heart using 12495-1-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 12495-1-AP (mu Crystallin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
human heart tissue were subjected to SDS PAGE followed by western blot with 12495-1-AP (mu Crystallin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IHC staining of human gliomas using 12495-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12495-1-AP (mu Crystallin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12495-1-AP (mu Crystallin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human gliomas using 12495-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12495-1-AP (mu Crystallin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12495-1-AP (mu Crystallin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of human gliomas using 12495-1-AP
Immunofluorescent analysis of (4% PFA) fixed human gliomas tissue using Mu Crystallin antibody (12495-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
Immunofluorescent analysis of (4% PFA) fixed human gliomas tissue using Mu Crystallin antibody (12495-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of human gliomas using 12495-1-AP
Immunofluorescent analysis of (4% PFA) fixed human gliomas tissue using Mu Crystallin antibody (12495-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
Immunofluorescent analysis of (4% PFA) fixed human gliomas tissue using Mu Crystallin antibody (12495-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
FC experiment of SH-SY5Y using 12495-1-AP
1X10^6 SH-SY5Y cells were intracellularly stained with 0.2 ug Anti-Human Mu Crystallin (12495-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
1X10^6 SH-SY5Y cells were intracellularly stained with 0.2 ug Anti-Human Mu Crystallin (12495-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
Y79 cells, Jurkat cells, human kidney tissue, human heart tissue
Positive IHC detected in
human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected in
human gliomas tissue
Positive FC (Intra) detected in
SH-SY5Y cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:1000-1:4000
Immunohistochemistry (IHC)
IHC : 1:50-1:500
Immunofluorescence (IF)-P
IF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)
FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
mu Crystallin(thiomorpholine-carboxylate dehydrogenase) is also named as THBP, CRYM, ketimine reductase and belongs to the ornithine cyclodeaminase family. This protein catalyzes the reduction of imine bonds in brain substrates that may include cystathionine ketimine (CysK) and lanthionine ketimine (LK). It is also involved in the regulation of the free intracellular concentration of triiodothyronine and access to its nuclear receptor.
Expression of CRYM in different rat organs during development and its decreased expression in degenerating pyramidal tracts in amyotrophic lateral sclerosis.
Y79 cells were subjected to SDS PAGE followed by western blot with 12495-1-AP (mu Crystallin antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of human kidney using 12495-1-AP
human kidney tissue were subjected to SDS PAGE followed by western blot with 12495-1-AP (mu Crystallin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 12495-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 12495-1-AP (mu Crystallin antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of human heart using 12495-1-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 12495-1-AP (mu Crystallin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human gliomas using 12495-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12495-1-AP (mu Crystallin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human gliomas using 12495-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12495-1-AP (mu Crystallin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF-P Figures
IF Staining of human gliomas using 12495-1-AP
Immunofluorescent analysis of (4% PFA) fixed human gliomas tissue using Mu Crystallin antibody (12495-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of human gliomas using 12495-1-AP
Immunofluorescent analysis of (4% PFA) fixed human gliomas tissue using Mu Crystallin antibody (12495-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
FC (INTRA) Figures
FC experiment of SH-SY5Y using 12495-1-AP
1X10^6 SH-SY5Y cells were intracellularly stained with 0.2 ug Anti-Human Mu Crystallin (12495-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
At Proteintech, we pride ourselves on our antibody quality, customer service and transparency. As such, we are comparing our antibodies with other vendors, enabling easy identification and comparisons of key data to help you choose the suitable antibody for your needs.
We have selected the top cited antibodies from these vendors for you to compare.
Proteintech
Mu Crystallin Polyclonal antibody
Catalog Number
12495-1-AP
Citations
3
Dilutions
WB : 1:1000-1:4000 IHC : 1:50-1:500 IF-P : 1:50-1:500 FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
Applications
WB, IHC, IF-P, FC (Intra), ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet