Mouse IgG2b isotype control Monoclonal antibody, PBS Only

Mouse IgG2b isotype control Monoclonal Antibody for WB, FC, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Megathura crenulata

Applications

WB, FC, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

11B8C4

Cat no : 66360-3-PBS

Synonyms

Mouse IgG2b, Mouse IgG2b isotype control

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Validation Data Gallery

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  WB analysis using 66360-3-Ig (same clone as 66360-3-PBS)

  Various lysates (50 ug/lane) were subjected to SDS PAGE followed by western blot with 66360-3-Ig (Mouse IgG2b isotype control antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66360-3-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 66360-3-Ig (same clone as 66360-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66360-3-Ig (Mouse IgG2b isotype control antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66360-3-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 66360-3-Ig (same clone as 66360-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66360-3-Ig (Mouse IgG2b isotype control antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66360-3-PBS in a different storage buffer formulation.

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  FC experiment of Jurkat using 66360-3-Ig (same clone as 66360-3-PBS)

  1X10^6 Jurkat cells were stained with 0.20ug Mouse IgG2b isotype control antibody (66360-3-Ig, red) or control antibody (blue). Fixed with 90% MeOH. This data was developed using the same antibody clone with 66360-3-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66360-3-PBS targets Mouse IgG2b isotype control in WB, FC, IHC, ELISA applications and shows reactivity with Megathura crenulata samples.

• Tested Reactivity: Megathura crenulata
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: Mouse IgG2b isotype control
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

This antibody is used as the isotype control of mouse IgG2b. It can be used as the isotype control in Flow Cytometry, Immuno-Precipitation and other experiments. The immunogen of this antibody is Keyhole Limpet Hemocyanin (KLH).