Mouse IgG2b isotype control Monoclonal antibody, PBS Only

Mouse IgG2b isotype control Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

megathura crenulata

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

11B8C4

Cat no : 66360-3-PBS

Synonyms

Mouse IgG2b

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Validation Data Gallery

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  WB analysis using 66360-3-Ig (same clone as 66360-3-PBS)

  Various lysates (50 ug/lane) were subjected to SDS PAGE followed by western blot with 66360-3-Ig (Mouse IgG2b isotype control antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66360-3-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 66360-3-Ig (same clone as 66360-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66360-3-Ig (Mouse IgG2b isotype control antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66360-3-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 66360-3-Ig (same clone as 66360-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66360-3-Ig (Mouse IgG2b isotype control antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66360-3-PBS in a different storage buffer formulation.

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  FC experiment of human PBMCs using 66360-3-Ig (same clone as 66360-3-PBS)

  1x10^6 human PBMCs were surface stained with 0.5 ug Mouse IgG2b isotype control Monoclonal antibody (66360-3-Ig, Clone: 11B8C4) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1) (red), or 0.5 ug Anti-Human CD20 (65085-1-Ig) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1) (green), or unstained (blue). Cells were not fixed. This data was developed using the same antibody clone with 66360-3-PBS in a different storage buffer formulation.

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Product Information

66360-3-PBS targets Mouse IgG2b isotype control in WB, IHC, ELISA applications and shows reactivity with megathura crenulata samples.

• Tested Reactivity: megathura crenulata
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: Mouse IgG2b isotype control
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

This antibody is used as the isotype control of mouse IgG2b. It can be used as the isotype control in Flow Cytometry, Immuno-Precipitation and other experiments. The immunogen of this antibody is Keyhole Limpet Hemocyanin (KLH).