CoraLite® Plus 647 Mouse IgG1 Isotype Control (1F8D3)

IgG1 Isotype Control Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1

Reactivity

Megathura crenulata

Applications

FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

1F8D3

Cat no : CL647-66360-1

Synonyms

Kappa light chain, Mouse IgG1, Mouse IgG1 isotype control

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Validation Data Gallery

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  FC experiment of HepG2 using CL647-66360-1

  1X10^6 HepG2 cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Mouse IgG1 isotype control (CL647-66360-1, Clone:1F8D3) (red), or 0.2 ug CoraLite® Plus 647 Lamin B1 antibody (CL647-66095, Clone: 3C10G12) (green), or unstained (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

Positive FC detected in

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-66360-1 targets IgG1 Isotype Control in FC applications and shows reactivity with Megathura crenulata samples.

• Tested Reactivity: Megathura crenulata
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: IgG1 Isotype Control
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

This antibody is used as the isotype control of mouse IgG1 (Kappa). It can be used as the isotype control in Flow Cytometry, Immuno-Precipitation and other experiments. The immunogen of this antibody is Keyhole Limpet Hemocyanin (KLH).