1X10^6 HepG2 cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Mouse IgG1 isotype control (CL647-66360-1, Clone:1F8D3) (red), or 0.2 ug CoraLite® Plus 647 Lamin B1 antibody (CL647-66095, Clone: 3C10G12) (green), or unstained (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).
1X10^6 HepG2 cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Mouse IgG1 isotype control (CL647-66360-1, Clone:1F8D3) (red), or 0.2 ug CoraLite® Plus 647 Lamin B1 antibody (CL647-66095, Clone: 3C10G12) (green), or unstained (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
CL647-66360-1 targets IgG1 Isotype Control in FC applications and shows reactivity with Megathura crenulata samples.
Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.
Background Information
This antibody is used as the isotype control of mouse IgG1 (Kappa). It can be used as the isotype control in Flow Cytometry, Immuno-Precipitation and other experiments. The immunogen of this antibody is Keyhole Limpet Hemocyanin (KLH).
Protocols
Product Specific Protocols
FC protocol for CL Plus 647 IgG1 Isotype Control antibody CL647-66360-1
1X10^6 HepG2 cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Mouse IgG1 isotype control (CL647-66360-1, Clone:1F8D3) (red), or 0.2 ug CoraLite® Plus 647 Lamin B1 antibody (CL647-66095, Clone: 3C10G12) (green), or unstained (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
IgG1 Isotype Control Monoclonal antibody
Catalog Number
CL647-66360-1
Citations
-
Dilutions
FC (INTRA) :
Applications
FC
Reactivity
megathura crenulata
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
