Mouse IgG1 isotype control Monoclonal antibody

Mouse IgG1 isotype control Monoclonal Antibody for WB, FC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

megathura crenulata and More (2)

Applications

WB, IHC, IF, FC , IP, CoIP, RIP, Neutralization, ELISA, Cell treatment

Conjugate

Unconjugated

Publications

18

CloneNo.

1F8D3

Cat no : 66360-1-Ig

Synonyms

Mouse IgG1, Kappa light chain

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• Validation Data Gallery
• Proteintech vs. Other Vendors

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  WB analysis using 66360-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66360-1-Ig (Mouse IgG1 isotype control antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 66360-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66360-1-Ig (Mouse IgG1 isotype control antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  FC experiment of human PBMCs using 66360-1-Ig

  1x10^6 human PBMCs were surface stained with 0.5 ug Mouse IgG1 isotype control Monoclonal antibody (66360-1-Ig, Clone: 1F8D3) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1) (red), or 0.5 ug Anti-Human CD3 (65151-1-Ig) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1) (green), or unstained (blue). Cells were not fixed.

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Flow Cytometry (FC): FC : 0.50 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 2 publications below
• FC: See 2 publications below
• CoIP: See 1 publications below
• RIP: See 2 publications below

Product Information

66360-1-Ig targets Mouse IgG1 isotype control in WB, IHC, IF, FC , IP, CoIP, RIP, Neutralization, ELISA, Cell treatment applications and shows reactivity with megathura crenulata samples.

• Tested Reactivity: megathura crenulata
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: Mouse IgG1 isotype control
• RRID: AB_2827991
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

This antibody is used as the isotype control of mouse IgG1 (Kappa). It can be used as the isotype control in Flow Cytometry, Immuno-Precipitation and other experiments. The immunogen of this antibody is Keyhole Limpet Hemocyanin (KLH).

Publications

Species	Application	Title
	CoIP	FASEB J; Bile acid restrained T cell activation explains cholestasis aggravated hepatitis B virus infection; Authors - Chujie Ding
	FC	J Immunol; Phagocytic Plasma Cells in Teleost Fish Provide Insights into the Origin and Evolution of B Cells in Vertebrates; Authors - Xue-Qing Han
	IP	Exp Cell Res; CBL aggravates Ang II-induced cardiac hypertrophy via the VHL/HIF-1α pathway.; Authors - Yuting Yang
	IHC	Am J Rhinol Allergy; Co-Expression and Localization of Angiotensin-Converting Enzyme-2 (ACE2) and the Transmembrane Serine Protease 2 (TMPRSS2) in Paranasal Ciliated Epithelium of Patients with Chronic Rhinosinusitis.; Authors - Tomohiro Kawasumi
		bioRxiv; Immunojanus Particles for low-volume and isolation-free unlabeled characterization of small Extracellular Vesicle in biofluids: Characterization of disease type by surface marker profiling; Authors - Sonu Kumar
		Biomedicines; Pro-Inflammatory Characteristics of Extracellular Vesicles in the Vitreous of Type 2 Diabetic Patients; Authors - Shengshuai Shan