Mouse IgG1 isotype control Monoclonal antibody

Mouse IgG1 isotype control Monoclonal Antibody for WB, FC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

megathura crenulata and More (2)

Applications

WB, IHC, IF, FC , IP, CoIP, RIP, Neutralization, ELISA, Cell treatment

Conjugate

Unconjugated

CloneNo.

1F8D3

Cat no : 66360-1-Ig

Synonyms

Mouse IgG1, Kappa light chain


Filter:
  • Western Blot (WB) analysis of various lysates using Mouse IgG1 isotype control Monoclonal antibody (66360-1-Ig)

    WB analysis using 66360-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66360-1-Ig (Mouse IgG1 isotype control antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using Mouse IgG1 isotype control Monoclonal antibody (66360-1-Ig)

    WB analysis using 66360-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66360-1-Ig (Mouse IgG1 isotype control antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of human PBMCs using Mouse IgG1 isotype control Monoclonal antibody (66360-1-Ig)

    FC experiment of human PBMCs using 66360-1-Ig

    1x10^6 human PBMCs were surface stained with 0.5 ug Mouse IgG1 isotype control Monoclonal antibody (66360-1-Ig, Clone: 1F8D3) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1) (red), or 0.5 ug Anti-Human CD3 (65151-1-Ig) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1) (green), or unstained (blue). Cells were not fixed.

"Mouse IgG1 isotype control Antibodies" Comparison

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Tested Applications

Positive WB detected in
Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Flow Cytometry (FC)FC : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66360-1-Ig targets Mouse IgG1 isotype control in WB, IHC, IF, FC , IP, CoIP, RIP, Neutralization, ELISA, Cell treatment applications and shows reactivity with megathura crenulata samples.

Tested Reactivity megathura crenulata
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide
Full Name Mouse IgG1 isotype control
Gene Symbol
Gene ID (NCBI)
RRIDAB_2827991
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

This antibody is used as the isotype control of mouse IgG1 (Kappa). It can be used as the isotype control in Flow Cytometry, Immuno-Precipitation and other experiments. The immunogen of this antibody is Keyhole Limpet Hemocyanin (KLH).

Protocols

Product Specific Protocols
WB protocol for Mouse IgG1 isotype control antibody 66360-1-IgDownload protocol
FC protocol for Mouse IgG1 isotype control antibody 66360-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
CoIP

FASEB J

Bile acid restrained T cell activation explains cholestasis aggravated hepatitis B virus infection

Authors - Chujie Ding
FC

J Immunol

Phagocytic Plasma Cells in Teleost Fish Provide Insights into the Origin and Evolution of B Cells in Vertebrates

Authors - Xue-Qing Han
IP

Exp Cell Res

CBL aggravates Ang II-induced cardiac hypertrophy via the VHL/HIF-1α pathway.

Authors - Yuting Yang
IHC

Am J Rhinol Allergy

Co-Expression and Localization of Angiotensin-Converting Enzyme-2 (ACE2) and the Transmembrane Serine Protease 2 (TMPRSS2) in Paranasal Ciliated Epithelium of Patients with Chronic Rhinosinusitis.

Authors - Tomohiro Kawasumi
RIP

Cell Biosci

PABPN1 regulates mRNA alternative polyadenylation to inhibit bladder cancer progression

Authors - Liang Chen

Stem Cell Res Ther

Menstrual blood-derived endometrial stem cells alleviate neuroinflammation by modulating M1/M2 polarization in cell and rat Parkinson's disease models

Authors - Han Li