Mouse IgG1 isotype control Monoclonal antibody
Mouse IgG1 isotype control Monoclonal Antibody for WB, FC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
megathura crenulata and More (2)
Applications
WB, IHC, IF, FC , IP, CoIP, RIP, Neutralization, ELISA, Cell treatment
Conjugate
Unconjugated
CloneNo.
1F8D3
Cat no : 66360-1-Ig
Synonyms
"Mouse IgG1 isotype control Antibodies" Comparison
View side-by-side comparison of Mouse IgG1 isotype control antibodies from other vendors to find the one that best suits your research needs.
Tested Applications
Positive WB detected in | |
Positive FC detected in | human PBMCs |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Flow Cytometry (FC) | FC : 0.50 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
66360-1-Ig targets Mouse IgG1 isotype control in WB, IHC, IF, FC , IP, CoIP, RIP, Neutralization, ELISA, Cell treatment applications and shows reactivity with megathura crenulata samples.
Tested Reactivity | megathura crenulata |
Cited Reactivity | human, mouse |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | Peptide |
Full Name | Mouse IgG1 isotype control |
Gene Symbol | |
Gene ID (NCBI) | |
RRID | AB_2827991 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
This antibody is used as the isotype control of mouse IgG1 (Kappa). It can be used as the isotype control in Flow Cytometry, Immuno-Precipitation and other experiments. The immunogen of this antibody is Keyhole Limpet Hemocyanin (KLH).
Protocols
Product Specific Protocols | |
---|---|
WB protocol for Mouse IgG1 isotype control antibody 66360-1-Ig | Download protocol |
FC protocol for Mouse IgG1 isotype control antibody 66360-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
FASEB J Bile acid restrained T cell activation explains cholestasis aggravated hepatitis B virus infection | ||
J Immunol Phagocytic Plasma Cells in Teleost Fish Provide Insights into the Origin and Evolution of B Cells in Vertebrates | ||
Exp Cell Res CBL aggravates Ang II-induced cardiac hypertrophy via the VHL/HIF-1α pathway. | ||
Am J Rhinol Allergy Co-Expression and Localization of Angiotensin-Converting Enzyme-2 (ACE2) and the Transmembrane Serine Protease 2 (TMPRSS2) in Paranasal Ciliated Epithelium of Patients with Chronic Rhinosinusitis. | ||
Cell Biosci PABPN1 regulates mRNA alternative polyadenylation to inhibit bladder cancer progression | ||
Stem Cell Res Ther Menstrual blood-derived endometrial stem cells alleviate neuroinflammation by modulating M1/M2 polarization in cell and rat Parkinson's disease models |