Mouse IgG1 isotype control Monoclonal antibody

Mouse IgG1 isotype control Monoclonal Antibody for FC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Megathura crenulata

Applications

WB, IP, IHC, FC, ELISA

Conjugate

Unconjugated

CloneNo.

1F8D3

Cat no : 66360-1-Ig

Synonyms

Kappa light chain, KLH, Mouse IgG1 isotype control


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Applications:
  • WB analysis using 66360-1-Ig

    WB analysis using 66360-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66360-1-Ig (Mouse IgG1 isotype control antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis using 66360-1-Ig

    WB analysis using 66360-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66360-1-Ig (Mouse IgG1 isotype control antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • FC experiment of K-562 using 66360-1-Ig

    FC experiment of K-562 using 66360-1-Ig

    1X10^6 K-562 cells were stained with 0.20ug Mouse IgG1 isotype control antibody (66360-1-Ig, red) and another control antibody (blue). Fixed with 90% MeOH.


Tested Applications

Positive WB detected in
Positive FC detected in

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

66360-1-Ig targets Mouse IgG1 isotype control in WB, IP, IHC, FC, ELISA applications and shows reactivity with Megathura crenulata samples.

Tested Reactivity Megathura crenulata
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide
Full Name Mouse IgG1 isotype control
Gene symbol
Gene ID (NCBI)
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

This antibody is used as the isotype control of mouse IgG1 (Kappa). It can be used as the isotype control in Flow Cytometry, Immuno-Precipitation and other experiments. The immunogen of this antibody is Keyhole Limpet Hemocyanin (KLH).

Protocols

Product Specific Protocols
WB protocol for Mouse IgG1 isotype control antibody 66360-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
IHC

Am J Rhinol Allergy

Co-Expression and Localization of Angiotensin-Converting Enzyme-2 (ACE2) and the Transmembrane Serine Protease 2 (TMPRSS2) in Paranasal Ciliated Epithelium of Patients with Chronic Rhinosinusitis.

Authors - Tomohiro Kawasumi
IP

Exp Cell Res

CBL aggravates Ang II-induced cardiac hypertrophy via the VHL/HIF-1α pathway.

Authors - Yuting Yang