• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Mouse Activated T Cell Panel

Host

Hamster / Rat

Reactivity

Mouse

Applications

FC

Conjugate

Various

Cat no : PK30013

Validation Data Gallery

Applications:
  • 1x10^6 ConA stimulated mouse splenocytes were stained with PK30013 Mouse Activated T Cell Panel. CD3+ cells are gated. Parent population: live cells. Cells were not fixed.

    1x10^6 ConA stimulated mouse splenocytes were stained with PK30013 Mouse Activated T Cell Panel. CD3+ cells are gated. Parent population: live cells. Cells were not fixed.

  • 1x10^6 ConA stimulated mouse splenocytes were stained with PK30013 Mouse Activated T Cell Panel. CD4 and CD8 expression on CD3+ cells is shown. Cells were not fixed.

    1x10^6 ConA stimulated mouse splenocytes were stained with PK30013 Mouse Activated T Cell Panel. CD4 and CD8 expression on CD3+ cells is shown. Cells were not fixed.

  • 1x10^6 ConA stimulated (right) or unstimulated (left) mouse splenocytes were stained with PK30013 Mouse Activated T Cell Panel. CD25 and CD69 expression on CD3+ CD4+ T lymphocytes is shown. Cells were not fixed.

    1x10^6 ConA stimulated (right) or unstimulated (left) mouse splenocytes were stained with PK30013 Mouse Activated T Cell Panel. CD25 and CD69 expression on CD3+ CD4+ T lymphocytes is shown. Cells were not fixed.

  • 1x10^6 ConA stimulated (right) or unstimulated (left) mouse splenocytes were stained with PK30013 Mouse Activated T Cell Panel. CD25 and CD69 expression on CD3+ CD8+ T lymphocytes is shown. Cells were not fixed.

    1x10^6 ConA stimulated (right) or unstimulated (left) mouse splenocytes were stained with PK30013 Mouse Activated T Cell Panel. CD25 and CD69 expression on CD3+ CD8+ T lymphocytes is shown. Cells were not fixed.

Product Information

T lymphocytes are immune cells that respond to foreign antigens by secreting cytokines (CD8+ Cytotoxic T  cells) and coordinating the immune response (CD4+ Helper T cells).

This panel is designed for the identification of CD3+ T lymphocytes into CD4+ T Lymphocyte and CD8+T  Lymphocyte subpopulations and the evaluation of these two populations for activation with CD69 and  CD25. The PE channel is left open.


Panel Components

Fluorochrome (ex/em) Catalog NoTargetCloneIsotypeDescription       

CoraLite Plus 647                 

(654 nm/674 nm)             

CL647-65060CD3ε145-2C11Armenian Hamster IgG      T cell marker

CoraLite Plus 405                

(399 nm/422 nm)                

CL405-65104CD4GK1.5            Rat IgG2bk           T helper cell marker

CoraLite 594                

(588 nm/604 nm)                

CL594-65069

CD8a53-6.7Rat IgG2ak            Cytotoxic T cell marker
CoraLite Plus 488                  

(493 nm/522 nm)                

CL488-65105CD69H1.2F3 Armenian Hamster IgGMarker of activation

CoraLite Plus 750                  

(755 nm/780 nm)                

CL750-65137CD25 PC61.5 Rat IgG1λ            

Marker of activation

Storage

Storage Buffer: PBS with 0.09% sodium azide.

Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. 

Cited in Article as

PK30013, Mouse Activated T Cell Panel, Proteintech, IL, USA