CoraLite® Plus 750 Anti-Mouse CD25 (PC61.5)

CD25 Monoclonal Antibody for FC

Host / Isotype

Rat / IgG1, lambda

Reactivity

Mouse

Applications

FC

Conjugate

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

PC61.5

Cat no : CL750-65137

Synonyms

CD25, Il2r, Il2ra, Ly 43

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Validation Data Gallery

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  FC experiment of mouse splenocytes using CL750-65137

  1X10^6 stimulated (dashed) or plate-bound Anti-CD3 and Anti-CD28 stimulated mouse splenocytes were surface stained with 0.5 ug CoraLite® Plus 750 Anti-Mouse CD25 (CL750-65137, Clone: PC61.5) (red), or 0.5 ug Control Antibody. Cells were not fixed.

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Tested Applications

• Positive FC detected in: plate-bound Anti-CD3 and Anti-CD28 stimulated mouse splenocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL750-65137 targets CD25 in FC applications and shows reactivity with Mouse samples.

• Tested Reactivity: Mouse
• Host / Isotype: Rat / IgG1, lambda
• Class: Monoclonal
• Type: Antibody
• Immunogen: N/A
• Full Name: interleukin 2 receptor, alpha chain
• GenBank Accession Number: BC114437
• Gene Symbol: CD25
• Gene ID (NCBI): 16184
• Conjugate: CoraLite® Plus 750 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 755 nm / 780 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Proliferation of T lymphocytes is triggered by the interaction of IL-2 with its specific receptor following T lymphocyte activation. The receptor for IL-2 has three forms, generated by different combinations of three different proteins, the alpha chain (IL-2R alpha), the beta chain (IL-2R beta), and the gamma chain (IL-2R gamma) (PMID: 8476561). IL-2R alpha (also known as CD25) is a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes. CD25 is also found on natural CD4+Foxp3+ Treg cells (PMID: 22585674).