Filter: AllWBIHCIF/ICC

WB analysis using 66092-1-Ig (same clone as 66092-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 66092-1-Ig (Melanophilin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66092-1-PBS in a different storage buffer formulation.
WB analysis of pig brain using 66092-1-Ig (same clone as 66092-1-PBS)
pig brain tissue were subjected to SDS PAGE followed by western blot with 66092-1-Ig (Melanophilin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66092-1-PBS in a different storage buffer formulation.
WB analysis of HEK-293 using 66092-1-Ig (same clone as 66092-1-PBS)
HEK-293 cells were subjected to SDS PAGE followed by western blot with 66092-1-Ig (Melanophilin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66092-1-PBS in a different storage buffer formulation.
WB analysis of PC-3 using 66092-1-Ig (same clone as 66092-1-PBS)
PC-3 cells were subjected to SDS PAGE followed by western blot with 66092-1-Ig (Melanophilin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66092-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 66092-1-Ig (same clone as 66092-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66092-1-Ig (Melanophilin antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66092-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 66092-1-Ig (same clone as 66092-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66092-1-Ig (Melanophilin antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66092-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 66092-1-Ig (same clone as 66092-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney slide using 66092-1-Ig (Melanophilin antibody at dilution of 1:50. This data was developed using the same antibody clone with 66092-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 66092-1-Ig (same clone as 66092-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney slide using 66092-1-Ig (Melanophilin antibody at dilution of 1:50. This data was developed using the same antibody clone with 66092-1-PBS in a different storage buffer formulation.
IHC staining of human skin cancer using 66092-1-Ig (same clone as 66092-1-PBS)
Immunohistochemical analysis of paraffin-embedded human skin cancer slide using 66092-1-Ig (Melanophilin antibody at dilution of 1:50. This data was developed using the same antibody clone with 66092-1-PBS in a different storage buffer formulation.
IHC staining of human skin cancer using 66092-1-Ig (same clone as 66092-1-PBS)
Immunohistochemical analysis of paraffin-embedded human skin cancer slide using 66092-1-Ig (Melanophilin antibody at dilution of 1:50. This data was developed using the same antibody clone with 66092-1-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 66092-1-Ig (same clone as 66092-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 66092-1-Ig (Melanophilin antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66092-1-PBS in a different storage buffer formulation.

MLPH Monoclonal antibody, PBS Only

MLPH Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 66092-1-PBS

Clone No.1A8D12

Review Product

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

SlaC2-a, SLAC2A, SLAC2 A, Melanophilin, Exophilin-3

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

66092-1-PBS targets MLPH in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity	human, pig
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	MLPH fusion protein Ag19286 Predict reactive species
Full Name	melanophilin
Calculated Molecular Weight	66 kDa
Observed Molecular Weight	66 kDa
GenBank Accession Number	BC001653
Gene Symbol	MLPH
Gene ID (NCBI)	79083
RRID	AB_11232039
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q9BV36
Storage Buffer	PBS Only
Storage Conditions	Store at -80°C.

Background Information

Melanophilin (MLPH) is a member of the exophilin subfamily of Rab effector proteins. MLPH is a modular protein, forming a ternary complex with the small Ras-related GTPase Rab27A in its GTP-bound form and the motor protein myosin Va. It regulates retention of melanosomes at the peripheral actin cytoskeleton of melanocytes, a process essential for normal mammalian pigmentation. Defects in MLPH are a cause of Griscelli syndrome type 3 (GS3), which is characterized by a silver-gray hair color and abnormal pigment distribution in the hair shaft.