MLPH Monoclonal antibody

MLPH Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

2

CloneNo.

1A8D12

Cat no : 66092-1-Ig

Synonyms

SlaC2-a, SLAC2A, SLAC2 A, Melanophilin, Exophilin-3

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Validation Data Gallery

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  WB analysis using 66092-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66092-1-Ig (Melanophilin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig brain using 66092-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 66092-1-Ig (Melanophilin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 66092-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66092-1-Ig (Melanophilin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 66092-1-Ig

  PC-3 cells were subjected to SDS PAGE followed by western blot with 66092-1-Ig (Melanophilin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 66092-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66092-1-Ig (Melanophilin antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 66092-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66092-1-Ig (Melanophilin antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human kidney using 66092-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 66092-1-Ig (Melanophilin antibody at dilution of 1:50.

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  IHC staining of human kidney using 66092-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 66092-1-Ig (Melanophilin antibody at dilution of 1:50.

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  IHC staining of human skin cancer using 66092-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skin cancer slide using 66092-1-Ig (Melanophilin antibody at dilution of 1:50.

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  IHC staining of human skin cancer using 66092-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skin cancer slide using 66092-1-Ig (Melanophilin antibody at dilution of 1:50.

• 

  IF Staining of HepG2 using 66092-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 66092-1-Ig (Melanophilin antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, pig brain tissue, PC-3 cells, Jurkat cells, human brain tissue
• Positive IHC detected in: human kidney tissue, human skin cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IF: See 1 publications below

Product Information

66092-1-Ig targets MLPH in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, pig samples.

• Tested Reactivity: human, pig
• Cited Reactivity: human, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: MLPH fusion protein Ag19286
• Full Name: melanophilin
• Calculated Molecular Weight: 66 kDa
• Observed Molecular Weight: 66 kDa
• GenBank Accession Number: BC001653
• Gene Symbol: MLPH
• Gene ID (NCBI): 79083
• RRID: AB_11232039
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Melanophilin (MLPH) is a member of the exophilin subfamily of Rab effector proteins. MLPH is a modular protein, forming a ternary complex with the small Ras-related GTPase Rab27A in its GTP-bound form and the motor protein myosin Va. It regulates retention of melanosomes at the peripheral actin cytoskeleton of melanocytes, a process essential for normal mammalian pigmentation. Defects in MLPH are a cause of Griscelli syndrome type 3 (GS3), which is characterized by a silver-gray hair color and abnormal pigment distribution in the hair shaft.

Publications

Species	Application	Title
rat	WB,IF	Int J Biol Macromol; A multifunctional polydopamine/genipin/alendronate nanoparticle licences fibrin hydrogels osteoinductive and immunomodulatory potencies for repairing bone defects; Authors - Wentao Shi
human	WB	Mol Med Rep; Knockdown of microRNA‑143‑5p by STTM technology affects eumelanin and pheomelanin production in melanocytes.; Authors - Shuhui Qi