MYOD1 Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

MYOD1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 18943-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 18943-1-AP (same clone as 18943-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 18943-1-AP (MYOD1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18943-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human heart using 18943-1-AP (same clone as 18943-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 18943-1-AP (MYOD1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18943-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 18943-1-AP (same clone as 18943-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 18943-1-AP (MYOD1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18943-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse skeletal muscle using 18943-1-AP (same clone as 18943-1-PBS)

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 18943-1-AP (MYOD1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18943-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat heart using 18943-1-AP (same clone as 18943-1-PBS)

  rat heart tissue were subjected to SDS PAGE followed by western blot with 18943-1-AP (MYOD1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18943-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse heart using 18943-1-AP (same clone as 18943-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 18943-1-AP (MYOD1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18943-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse embryo using 18943-1-AP (same clone as 18943-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 18943-1-AP (MYOD1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18943-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse embryo using 18943-1-AP (same clone as 18943-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 18943-1-AP (MYOD1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18943-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse embryo using 18943-1-AP (same clone as 18943-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 18943-1-AP (MYOD1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18943-1-PBS in a different storage buffer formulation.

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Product Information

18943-1-PBS targets MYOD1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MYOD1 fusion protein Ag13512
• Full Name: myogenic differentiation 1
• Calculated Molecular Weight: 320 aa, 35 kDa
• Observed Molecular Weight: 35-45 kDa
• GenBank Accession Number: BC064493
• Gene Symbol: MYOD1
• Gene ID (NCBI): 4654
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

MYOD1, also named as BHLHC1 or MYF3, is a 320 amino acid protein, which promotes the transcriptional activity of MYOD1 through its CDK9-mediated phosphorylation. This phosphorylation promotes its function in muscle differentiation. MYOD1 acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation. MYOD1 together with MYF5 and MYOG, co-occupies muscle-specific gene promoter core region during myogenesis. Induces fibroblasts to differentiate into myoblasts. he calcualted molecular weight of MYOD1 is 34 kDa, but modified MYOD1 is about 45 kDa. (PMID: 12037670)