- Featured Product
- KD/KO Validated
MYOD1 Polyclonal antibody
MYOD1 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (7)
Applications
WB, IHC, IF, ChIP, ELISA
Conjugate
Unconjugated
Cat no : 18943-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse heart tissue, human heart tissue, mouse skeletal muscle tissue, rat heart tissue |
| Positive IHC detected in | mouse embryo tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 77 publications below |
| IHC | See 10 publications below |
| IF | See 25 publications below |
| ChIP | See 1 publications below |
Product Information
18943-1-AP targets MYOD1 in WB, IHC, IF, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig, rabbit, chicken, zebrafish, sheep, goat, duck |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | MYOD1 fusion protein Ag13512 |
| Full Name | myogenic differentiation 1 |
| Calculated Molecular Weight | 320 aa, 35 kDa |
| Observed Molecular Weight | 35-45 kDa |
| GenBank Accession Number | BC064493 |
| Gene Symbol | MYOD1 |
| Gene ID (NCBI) | 4654 |
| RRID | AB_10603467 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MYOD1, also named as BHLHC1 or MYF3, is a 320 amino acid protein, which promotes the transcriptional activity of MYOD1 through its CDK9-mediated phosphorylation. This phosphorylation promotes its function in muscle differentiation. MYOD1 acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation. MYOD1 together with MYF5 and MYOG, co-occupies muscle-specific gene promoter core region during myogenesis. Induces fibroblasts to differentiate into myoblasts. he calcualted molecular weight of MYOD1 is 34 kDa, but modified MYOD1 is about 45 kDa. (PMID: 12037670)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MYOD1 antibody 18943-1-AP | Download protocol |
| IHC protocol for MYOD1 antibody 18943-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Sci Adv Bioactive fiber-reinforced hydrogel to tailor cell microenvironment for structural and functional regeneration of myotendinous junction | ||
Nat Commun The transcription factor NF-Y participates to stem cell fate decision and regeneration in adult skeletal muscle. | ||
J Cachexia Sarcopenia Muscle circRNAome profiling reveals circFgfr2 regulates myogenesis and muscle regeneration via a feedback loop. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Charlotte (Verified Customer) (07-23-2024) | I reveal MyoD1 in C2C12 cells that differentiate into myoblast lineage upon stimulation. Antibodies were diluted in 5% milk TBST. The upper band is the expected one. I guess the lower one (< 35kDa) is aspecific
 |
FH Cassandra (Verified Customer) (02-04-2021) | This antibody worked quite well in my IF staining. I used 1 to 100 antibody dilution.
 |
