MYO1G Recombinant antibody, PBS Only

MYO1G Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240503E1

Cat no : 83556-5-PBS

Synonyms

Unconventional myosin-Ig, myosin IG, Minor histocompatibility antigen HA-2, HA 2, 240503E1

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Validation Data Gallery

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  WB analysis using 83556-5-RR (same clone as 83556-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83556-5-RR (MYO1G antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83556-5-PBS in a different storage buffer formulation.

• 

  IF Staining of Jurkat using 83556-5-RR (same clone as 83556-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using MYO1G antibody (83556-5-RR, Clone: 240503E1 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83556-5-PBS in a different storage buffer formulation.

• 

  IF Staining of Jurkat using 83556-5-RR (same clone as 83556-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using MYO1G antibody (83556-5-RR, Clone: 240503E1 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83556-5-PBS in a different storage buffer formulation.

• 

  FC experiment of Jurkat using 83556-5-RR (same clone as 83556-5-PBS)

  1x10^6 Jurkat cells were intracellularly stained with 0.25 ug MYO1G Recombinant antibody (83556-5-RR, Clone:240503E1) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83556-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83556-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83556-5-RR against Human MYO1G were performed. The affinity constant is 1.03 nM.

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Product Information

The immunogen of 83556-5-PBS is MYO1G Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: MYO1G fusion protein Ag22698
• Full Name: myosin IG
• Calculated Molecular Weight: 1018 aa, 116 kDa
• Observed Molecular Weight: 110-120 kDa
• GenBank Accession Number: BC113544
• Gene Symbol: MYO1G
• Gene ID (NCBI): 64005
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

MYO1G is a plasma membrane-associated class I myosin that is abundant in T and B lymphocytes and mast cells and expressed specifically in the haematopoietic system. Class I myosins have been implicated in various cellular processes relying on actin-dependent membrane dynamics such as endocytosis, secretion, adhesion, motility and regulation of mechanosensitive channels (PMID: 19968988). MYO1G localizes to the plasma membrane linked to PIP2 and PIP3, is particularly enriched at cell-surface microvilli, and associates in an ATP-releasable manner to the actin cytoskeleton (PMID: 24310084). MYO1G can be detected as about 110-120 kDa.