CoraLite® Plus 750-conjugated MYL7 Recombinant antibody

MYL7 Uni-rAb^TM Recombinant Antibody for WB, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra)

Conjugate

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

2C5

Cat no : CL750-81570

Synonyms

MYLC2A, MYL7/MLC2a, MYL2A, MLC-2a, MLC2a

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Validation Data Gallery

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  WB analysis using CL750-81570

  Various lysates were subjected to SDS PAGE followed by western blot with CL750-81570 (MYL7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  FC experiment of C2C12 using CL750-81570

  1x10^6 C2C12 cells were intracellularly stained with 0.4 ug CoraLite® Plus 750-conjugated MYL7 Recombinant antibody (CL750-81570, Clone:2C5)(red), or 0.4 ug Isotype Control (CL750-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: rat heart tissue, mouse heart tissue
• Positive FC (Intra) detected in: C2C12 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL750-81570 targets MYL7 in WB, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: MYL7 fusion protein Ag11058
• Full Name: myosin, light chain 7, regulatory
• Calculated Molecular Weight: 175 aa, 19 kDa
• Observed Molecular Weight: 19 kDa
• GenBank Accession Number: BC027915
• Gene Symbol: MYL7
• Gene ID (NCBI): 58498
• Conjugate: CoraLite® Plus 750 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 755 nm / 780 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

MYL7, also known as myosin light chain 2a (MLC2a), is essential for heart development. The expression of MYL7 in heart is predominatantly restricted to the atrium. So its expression has been considered as useful marker for atrial cardiomyocytes.