MYL7 Recombinant antibody

MYL7 Uni-rAb^TM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat, human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2C5

Cat no : 81570-1-RR

Synonyms

MLC 2a, MLC2a, MYL2A, MYL7, MYLC2A, Myosin light chain 2a

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Validation Data Gallery

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  WB analysis using 81570-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 81570-1-RR (MYL7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse heart using 81570-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 81570-1-RR (MYL7 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 81570-1-RR

  Biolayer interferometry (BLl) kinetic assays of 81570-1-RR against Human MYL7 were performed. The affinity constant is 0.25 nM.

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Tested Applications

• Positive WB detected in: mouse heart tissue, rat heart tissue
• Positive IHC detected in: mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

81570-1-RR targets MYL7 in WB, IHC, ELISA applications and shows reactivity with mouse, rat, human samples.

• Tested Reactivity: mouse, rat, human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: MYL7 fusion protein Ag11058
• Full Name: myosin, light chain 7, regulatory
• Calculated Molecular Weight: 175 aa, 19 kDa
• Observed Molecular Weight: 19 kDa
• GenBank Accession Number: BC027915
• Gene Symbol: MYL7
• Gene ID (NCBI): 58498
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MYL7, also known as myosin light chain 2a (MLC2a), is essential for heart development. The expression of MYL7 in heart is predominatantly restricted to the atrium. So its expression has been considered as useful marker for atrial cardiomyocytes.