MYL7 Recombinant antibody, PBS Only

MYL7 Uni-rAb^TM Recombinant Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat, human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2C5

Cat no : 81570-1-PBS

Synonyms

MLC 2a, MLC2a, MYL2A, MYL7, MYLC2A, Myosin light chain 2a

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Validation Data Gallery

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  WB analysis using 81570-1-RR (same clone as 81570-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 81570-1-RR (MYL7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81570-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 81570-1-RR (same clone as 81570-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 81570-1-RR (MYL7 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81570-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 81570-1-PBS

  Biolayer interferometry (BLl) kinetic assays of 81570-1-RR against Human MYL7 were performed. The affinity constant is 0.25 nM.

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Product Information

81570-1-PBS targets MYL7 in WB, IHC, Indirect ELISA applications and shows reactivity with mouse, rat, human samples.

• Tested Reactivity: mouse, rat, human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: MYL7 fusion protein Ag11058
• Full Name: myosin, light chain 7, regulatory
• Calculated Molecular Weight: 175 aa, 19 kDa
• Observed Molecular Weight: 19 kDa
• GenBank Accession Number: BC027915
• Gene Symbol: MYL7
• Gene ID (NCBI): 58498
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

MYL7, also known as myosin light chain 2a (MLC2a), is essential for heart development. The expression of MYL7 in heart is predominatantly restricted to the atrium. So its expression has been considered as useful marker for atrial cardiomyocytes.