CoraLite®594-conjugated MYL3 Monoclonal antibody

MYL3 Monoclonal Antibody for IF/ICC, IF-P

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

IF/ICC, IF-P

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2E4B2

Cat no : CL594-66286

Synonyms

Myosin light chain 3, Myosin light chain 1, slow-twitch muscle B/ventricular isoform, MYL 3, MLC-lV/sb, MLC1V

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Validation Data Gallery

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  IF Staining of mouse skeletal muscle using CL594-66286

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using CL594-66286 (MYL3 antibody) at dilution of 1:100.

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  IF Staining of C2C12 using CL594-66286

  Immunofluorescent analysis of (-20°C Ethanol) fixed C2C12 cells using CL594-66286 (MYL3 antibody) at dilution of 1:100.

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Tested Applications

• Positive IF-P detected in: mouse skeletal muscle tissue
• Positive IF/ICC detected in: C2C12 cells

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66286 targets MYL3 in IF/ICC, IF-P applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: MYL3 fusion protein Ag24535
• Full Name: myosin, light chain 3, alkali; ventricular, skeletal, slow
• Calculated Molecular Weight: 22 kDa
• Observed Molecular Weight: 22-25 kDa
• GenBank Accession Number: BC009790
• Gene Symbol: MYL3
• Gene ID (NCBI): 4634
• RRID: AB_2883551
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

MYL3, also named as MLC1v, is an essential light chain of myosin that is associated with muscle contraction. It is expressed in ventricular and slow skeletal muscle. MYL3 may serve as a target for caspase-3 in dying cardiomyocytes. Mutations of MYL3 gene cause hypertrophic cardiomyopathy. MYL3 has been identified as potential serum biomarker for drug induced myotoxicity. Great increase in MYL3 serum concentration has been observed in rats with cardiac and skeletal muscle injury. (PMID:21685905)