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Tested Applications
| Positive WB detected in | human skeletal muscle tissue, mouse heart tissue, human heart tissue, pig heart tissue, rat heart tissue |
| Positive IHC detected in | human heart tissue, human skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
Product Information
66286-1-Ig targets MYL3 in WB, IHC, ELISA applications and shows reactivity with human, rat, mouse, pig samples.
| Tested Reactivity | human, rat, mouse, pig |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | MYL3 fusion protein Ag24535 Predict reactive species |
| Full Name | myosin, light chain 3, alkali; ventricular, skeletal, slow |
| Calculated Molecular Weight | 22 kDa |
| Observed Molecular Weight | 22-25 kDa |
| GenBank Accession Number | BC009790 |
| Gene Symbol | MYL3 |
| Gene ID (NCBI) | 4634 |
| RRID | AB_2881669 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P08590 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MYL3, also named as MLC1v, is an essential light chain of myosin that is associated with muscle contraction. It is expressed in ventricular and slow skeletal muscle. MYL3 may serve as a target for caspase-3 in dying cardiomyocytes. Mutations of MYL3 gene cause hypertrophic cardiomyopathy. MYL3 has been identified as potential serum biomarker for drug induced myotoxicity. Great increase in MYL3 serum concentration has been observed in rats with cardiac and skeletal muscle injury. (PMID:21685905)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MYL3 antibody 66286-1-Ig | Download protocol |
| IHC protocol for MYL3 antibody 66286-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Antioxidants (Basel) Ganoderma lucidum Spore Powder Alleviates Metabolic-Associated Fatty Liver Disease by Improving Lipid Accumulation and Oxidative Stress via Autophagy | ||
Transl Oncol ACAT1 suppresses clear cell renal cell carcinoma progression by AMPK mediated fatty acid metabolism |
