MYCN Recombinant antibody, PBS Only (Capture)

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  WB analysis using 84886-3-RR (same clone as 84886-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84886-3-RR (MYCN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84886-3-PBS in a different storage buffer formulation.

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  WB analysis of human placenta using 84886-3-RR (same clone as 84886-3-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 84886-3-RR (MYCN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84886-3-PBS in a different storage buffer formulation.

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  FC experiment of SH-SY5Y using 84886-3-RR (same clone as 84886-3-PBS)

  1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug MYCN Recombinant antibody (84886-3-RR, Clone:242406D8) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 84886-3-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01659-2

  Cytometric bead array standard curve of MP01659-2, MYCN Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84886-3-PBS. Detection antibody: 84886-2-PBS. Standard: Ag27224. Range: 1.56-100 ng/mL.

MYCN Uni-rAb^TM Recombinant Antibody for WB, FC (Intra), Cytometric bead array, Indirect ELISA

Cat No. 84886-3-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), Cytometric bead array, Indirect ELISA

Synonyms

N-myc proto-oncogene protein, N myc proto oncogene protein, N myc, MODED, bHLHe37

Formulation:  PBS Only

PBS and Azide

PBS Only

Conjugate:  Unconjugated

Size/Concentration: 

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Product Information

84886-3-PBS targets MYCN as part of a matched antibody pair:

MP01659-2: 84886-3-PBS capture and 84886-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: MYCN fusion protein Ag27224
• Full Name: v-myc myelocytomatosis viral related oncogene, neuroblastoma derived (avian)
• Calculated Molecular Weight: 50 kDa
• Observed Molecular Weight: ~65 kDa
• GenBank Accession Number: BC002712
• Gene Symbol: MYCN
• Gene ID (NCBI): 4613
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P04198
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

MYCN, also named as BHLHE37 and NMYC, is a transcription factor. It is primarily expressed in normal developing embryos and is thought to be critical in brain and other neural development. It is often amplified in human neuroblastomas. IR34A can suppress MYCN expression, it suggest MYCN has a role in cell growth. The expression of the MCYN is upregulated in a variety of human tumors, most frequently neuroblastoma, where the level of expression appears to increase as the tumor progresses. The calculated molecuar weight of MYCN is 50 kDa, but Phosphorylated MYCN is about 65-70 kDa. MYCN exsits two isoforms and MV of isoform is respectively 65 kDa and 45 kDa. (PMID: 19615087 )