MYB/c-Myb Polyclonal antibody, PBS Only

• 

  WB analysis using 17800-1-AP (same clone as 17800-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 17800-1-AP (MYB/c-Myb antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17800-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 17800-1-AP (same clone as 17800-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 17800-1-AP (MYB/c-Myb antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17800-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 17800-1-AP (same clone as 17800-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17800-1-AP (MYB/c-Myb antibody at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17800-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HL-60 using 17800-1-AP (same clone as 17800-1-PBS)

  IP result of anti-MYB/c-Myb (IP:17800-1-AP, 4ug; Detection:17800-1-AP 1:300) with HL-60 cells lysate 4000ug. This data was developed using the same antibody clone with 17800-1-PBS in a different storage buffer formulation.

MYB/c-Myb Polyclonal Antibody for WB, IP, Indirect ELISA

Cat No. 17800-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, Indirect ELISA

Synonyms

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Product Information

17800-1-PBS targets MYB/c-Myb in WB, IP, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MYB/c-Myb fusion protein Ag12021
• Full Name: v-myb myeloblastosis viral oncogene homolog (avian)
• Calculated Molecular Weight: 640 aa, 72 kDa
• Observed Molecular Weight: 40-50 kDa, 72-80 kDa
• GenBank Accession Number: BC064955
• Gene Symbol: c-Myb
• Gene ID (NCBI): 4602
• RRID: AB_2148029
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P10242
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

The MYB protein is a transcription factor that plays a key role in regulating the proliferation/differentiation of progenitor cells in bone marrow, colonic crypts, and neurogenic niches [PMID:18574464]. It is involved in the regulation of epithelial-to-mesenchymal transition (EMT) and invasion in neuroblastoma, colon carcinoma, and embryonic kidney cells through the upregulation of the transcription repressor Slug [PMID:20622260]. It also a DNA-binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3', and plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells. MYB exists some isoforms with MV 85, 75, 72, 68, 63 and 40 kDa.