MYB/c-Myb Polyclonal antibody

MYB/c-Myb Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IF, RIP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 17800-1-AP

Synonyms

c myb_CDS, Cmyb, c-Myb, efg, MYB, Proto oncogene c Myb, Transcriptional activator Myb



Tested Applications

Positive WB detected inHL-60 cells, Jurkat cells, MOLT-4 cells
Positive IP detected inHL-60 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17800-1-AP targets MYB/c-Myb in WB, IP, IF, RIP, CoIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MYB/c-Myb fusion protein Ag12021
Full Name v-myb myeloblastosis viral oncogene homolog (avian)
Calculated Molecular Weight 640 aa, 72 kDa
Observed Molecular Weight40-50 kDa, 72-80 kDa
GenBank Accession NumberBC064955
Gene Symbol c-Myb
Gene ID (NCBI) 4602
RRIDAB_2148029
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The MYB protein is a transcription factor that plays a key role in regulating the proliferation/differentiation of progenitor cells in bone marrow, colonic crypts, and neurogenic niches [PMID:18574464]. It is involved in the regulation of epithelial-to-mesenchymal transition (EMT) and invasion in neuroblastoma, colon carcinoma, and embryonic kidney cells through the upregulation of the transcription repressor Slug [PMID:20622260]. It also a DNA-binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3', and plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells. MYB exists some isoforms with MV 85, 75, 72, 68, 63 and 40 kDa.

Protocols

Product Specific Protocols
WB protocol for MYB/c-Myb antibody 17800-1-APDownload protocol
IP protocol for MYB/c-Myb antibody 17800-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

NLRP3 phosphorylation in its LRR domain critically regulates inflammasome assembly.

Authors - Tingting Niu
mouseWB

Nat Commun

Tcf1 and Lef1 provide constant supervision to mature CD8+ T cell identity and function by organizing genomic architecture.

Authors - Qiang Shan
humanWB,CoIP

RNA Biol

SND1 acts as an anti-apoptotic factor via regulating the expression of lncRNA UCA1 in hepatocellular carcinoma.

Authors - Xiaoteng Cui
humanIF,WB

Front Endocrinol (Lausanne)

KRT17 from skin cells with high glucose stimulation promotes keratinocytes proliferation and migration

Authors - Peng Zhou
humanWB

Front Med (Lausanne)

Lidocaine and Bupivacaine Downregulate MYB and DANCR lncRNA by Upregulating miR-187-5p in MCF-7 Cells.

Authors - Chiao-Yi Lin
mouseWB

Cancer Genomics Proteomics

Myb Repression Mediates Stat5b-knockdown-induced Apoptosis and Inhibits Proliferation of Glioblastoma Stem Cells

Authors - Chiami Moyama