Tested Applications
Positive WB detected in | MCF-7 cells, Jurkat cells, HepG2 cells, HeLa cells |
Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:2000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:125-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
84328-1-RR targets MTMR4 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | MTMR4 fusion protein Ag4310 Predict reactive species |
Full Name | myotubularin related protein 4 |
Calculated Molecular Weight | 1195 aa, 133 kDa |
Observed Molecular Weight | 160-170 kDa |
GenBank Accession Number | BC035609 |
Gene Symbol | MTMR4 |
Gene ID (NCBI) | 9110 |
RRID | AB_3671869 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purfication |
UNIPROT ID | Q9NYA4 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Myotubularin-related protein-4 (MTMR4), also named FYVE-DSP2, is a member of the myotubularin family of phosphoinositide phosphatases that selectively degrade the 3-position phosphate from the inositol ring of PtdIns(3)P and phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2) (PMID: 31543504). MTMR4 knockdown markedly suppressed the motility, fusion, and fission of PI(3)P- enriched structures, resulting in decreases in late endosomes, autophagosomes, and lysosomes, and enlargement of PI(3)P- enriched early and late endosomes(PMID: 29962048). MTMR4 is an intracellular protein with a calculated molecular mass of 133 kDa, and detected molecular weight in the papers is about 160-170 kDa, which may be due to post-translational modification (PMID: 19125695, PMID: 20736309).
Protocols
Product Specific Protocols | |
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WB protocol for MTMR4 antibody 84328-1-RR | Download protocol |
IF protocol for MTMR4 antibody 84328-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |