MTMR4 Recombinant antibody

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  WB analysis of Jurkat using 84328-1-RR

  WB result of MTMR4 antibody (84328-1-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MTMR4 transfected Jurkat cells.

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  WB analysis using 84328-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84328-1-RR (MTMR4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 84328-1-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MTMR4 antibody (84328-1-RR, Clone: 241304E11 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 84328-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84328-1-RR against Human MTMR4 were performed. The affinity constant is 2.06 nM.

• Featured Product
• KD/KO Validated

MTMR4 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 84328-1-RR

Clone No.241304E11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

KIAA0647, FYVE-DSP2, FYVE DSP2, FYVE domain-containing dual specificity protein phosphatase 2, EC:3.1.3.48

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Tested Applications

• Positive WB detected in: MCF-7 cells, Jurkat cells, HepG2 cells, HeLa cells
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84328-1-RR targets MTMR4 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: MTMR4 fusion protein Ag4310
• Full Name: myotubularin related protein 4
• Calculated Molecular Weight: 1195 aa, 133 kDa
• Observed Molecular Weight: 160-170 kDa
• GenBank Accession Number: BC035609
• Gene Symbol: MTMR4
• Gene ID (NCBI): 9110
• RRID: AB_3671869
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q9NYA4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Myotubularin-related protein-4 (MTMR4), also named FYVE-DSP2, is a member of the myotubularin family of phosphoinositide phosphatases that selectively degrade the 3-position phosphate from the inositol ring of PtdIns(3)P and phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2) (PMID: 31543504). MTMR4 knockdown markedly suppressed the motility, fusion, and fission of PI(3)P- enriched structures, resulting in decreases in late endosomes, autophagosomes, and lysosomes, and enlargement of PI(3)P- enriched early and late endosomes(PMID: 29962048). MTMR4 is an intracellular protein with a calculated molecular mass of 133 kDa, and detected molecular weight in the papers is about 160-170 kDa, which may be due to post-translational modification (PMID: 19125695, PMID: 20736309).