CoraLite® Plus 488-conjugated MTH1 Monoclonal antibody

MTH1 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2D7G4

Cat no : CL488-67443

Synonyms

NUDT1, EC:3.6.1.-, 8-oxo-dGTPase, 8 oxo dGTPase, 7,8-dihydro-8-oxoguanine triphosphatase

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Validation Data Gallery

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  IF Staining of HepG2 using CL488-67443

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 MTH1 antibody (CL488-67443, Clone: 2D7G4 ) at dilution of 1:200.

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  FC experiment of Jurkat using CL488-67443

  1X10^6 Jurkat cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human MTH1 (CL488-67443, Clone:2D7G4) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: Jurkat cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67443 targets MTH1 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: MTH1 fusion protein Ag9802
• Full Name: nudix (nucleoside diphosphate linked moiety X)-type motif 1
• Calculated Molecular Weight: 179aa,20 kDa; 197aa,23 kDa
• Observed Molecular Weight: 19 kDa
• GenBank Accession Number: BC014618
• Gene Symbol: MTH1
• Gene ID (NCBI): 4521
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

MTH1, also known as NUDT1 or 8-Oxo-dGTPase, is an important repair enzyme that prevents oxidative stress-induced DNA damage through hydrolyzing mutagenic 8-oxo-dGTP into 8-oxo-dGMP. MTH1 is expressed in postmitotic neurons as well as in proliferative tissues, and it is localized both in the mitochondria and nucleus. MTH1 might be a useful marker of oxidative stress and its level increased upon oxidative stress. Increased expression of MTH1 were also found in various cancer tissues and neurodegenerative diseases.