MTF2 Recombinant antibody

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  WB analysis using 82944-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82944-1-RR (MTF2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 82944-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MTF2 antibody (82944-1-RR, Clone: 230138D10 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  FC experiment of HEK-293 using 82944-1-RR

  1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug MTF2 Recombinant antibody (82944-1-RR, Clone:230138D10) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  Affinity and Kinetic Characterization of 82944-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82944-1-RR against Human MTF2 were performed. The affinity constant is 0.398 nM.

MTF2 Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 82944-1-RR

Clone No.230138D10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

MTF 2, Metal-response element-binding transcription factor 2, Metal-response element DNA-binding protein M96, M96, hPCl2

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Tested Applications

• Positive WB detected in: HEK-293T cells, Jurkat cells, HeLa cells, HepG2 cells
• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82944-1-RR targets MTF2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: MTF2 fusion protein Ag9384
• Full Name: metal response element binding transcription factor 2
• Calculated Molecular Weight: 536 aa, 61 kDa
• Observed Molecular Weight: 55 kDa, 60 kDa, 75 kDa
• GenBank Accession Number: BC010013
• Gene Symbol: MTF2
• Gene ID (NCBI): 22823
• RRID: AB_3670687
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9Y483
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MTF2, also named as Metal-response element-binding transcription factor 2, is a 593 amino acid protein. It exists several isoforms and the range of the molecular weight of isoforms are about 55-60 kDa and 29 kDa. MTF2 contains 2 PHD-type zinc fingers and belongs to the Polycomblike family. MTF2 acts by binding to H3K36me3, a mark for transcriptional activation, and recruiting the PRC2 complex, leading to enhance PRC2 H3K27me3 methylation activity and regulates the transcriptional networks during embryonic stem cell self-renewal and differentiation.