CoraLite® Plus 750-conjugated MTA3 Recombinant antibody

MTA3 Recombinant Antibody for WB, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra)

Conjugate

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

230350D12

Cat no : CL750-83042-3

Synonyms

mta1, MTA 3, Metastasis-associated protein MTA3, 230350D12

---

Validation Data Gallery

• 

  WB analysis using CL750-83042-3

  Various lysates were subjected to SDS PAGE followed by western blot with CL750-83042-3 (MTA3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  FC experiment of U-251 using CL750-83042-3

  1x10^6 U-251 cells were intracellularly stained with 0.8 ug Coralite® Plus 750 Mta3 Recombinant Antibody (CL750-83042-3, Clone:230350D12)(red), or 0.8 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

---

Tested Applications

• Positive WB detected in: MCF-7 cells, Raji cells
• Positive FC (Intra) detected in: U-251 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL750-83042-3 targets MTA3 in WB, FC (Intra) applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: MTA3 fusion protein Ag6400
• Full Name: metastasis associated 1 family, member 3
• Calculated Molecular Weight: 68 kDa
• Observed Molecular Weight: 62 kDa, 68 kDa
• GenBank Accession Number: BC053631
• Gene Symbol: MTA3
• Gene ID (NCBI): 57504
• Conjugate: CoraLite® Plus 750 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 755 nm / 780 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

The metastasis-associated (MTA) family contains three members, MTA1, MTA2 and MTA3 (PMID:12167865).Metastasis-associated protein 3 (MTA3) is a cell-type specific component of the Mi-2-NURD transcriptional co-repressor complex. It has a role in maintenance of the normal epithelial architecture through the repression of SNAI1 transcription in a histone deacetylase-dependent manner, and thus the regulation of E-cadherin levels.