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MRPL40 Recombinant antibody, PBS Only

MRPL40 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240506A9

Cat no : 83515-4-PBS

Synonyms

MRP-L40, MRP L40, MRP L22, Large ribosomal subunit protein mL40, L40mt

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MRPL40 Recombinant antibody (83515-4-RR)

    WB analysis using 83515-4-RR (same clone as 83515-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83515-4-RR (MRPL40 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83515-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using MRPL40 Recombinant antibody (83515-4-RR)

    IHC staining of human intrahepatic cholangiocarcinoma using 83515-4-RR (same clone as 83515-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83515-4-RR (MRPL40 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83515-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using MRPL40 Recombinant antibody (83515-4-RR)

    IF Staining of HeLa using 83515-4-RR (same clone as 83515-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MRPL40 antibody (83515-4-RR, Clone: 240506A9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83515-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83515-4-RR

    Affinity and Kinetic Characterization of 83515-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83515-4-RR against Human MRPL40 were performed. The affinity constant is 1.56 nM.

Product Information

The immunogen of 83515-4-PBS is MRPL40 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MRPL40 fusion protein Ag10341
Full Name mitochondrial ribosomal protein L40
Calculated Molecular Weight 206 aa, 24 kDa
Observed Molecular Weight18-24 kDa
GenBank Accession NumberBC009707
Gene Symbol MRPL40
Gene ID (NCBI) 64976
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

MRPL40, also known as NLVCF and URIM, belongs to the mitochondrion-specific ribosomal protein mL40 family. MRPL40 encodes the mitochondrial ribosomal protein L40 for the larger 39S subunit of mitochondrial ribosomes. MRPL40 is widely expressed in human organs and tissues, including the heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, ovary, small intestine, colon, peripheral blood lymphocyte (PBL), and testis. MRPL40 is also expressed in human fetuses and mouse embryos, suggesting its important role in fetal development (PMID: 36891938).