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MRPL40 Recombinant antibody

MRPL40 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240506A9

Cat no : 83515-4-RR

Synonyms

MRP-L40, MRP L40, MRP L22, Large ribosomal subunit protein mL40, L40mt

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MRPL40 Recombinant antibody (83515-4-RR)

    WB analysis using 83515-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83515-4-RR (MRPL40 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using MRPL40 Recombinant antibody (83515-4-RR)

    IHC staining of human intrahepatic cholangiocarcinoma using 83515-4-RR

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83515-4-RR (MRPL40 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using MRPL40 Recombinant antibody (83515-4-RR)

    IF Staining of HeLa using 83515-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MRPL40 antibody (83515-4-RR, Clone: 240506A9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 83515-4-RR

    Affinity and Kinetic Characterization of 83515-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83515-4-RR against Human MRPL40 were performed. The affinity constant is 1.56 nM.

Tested Applications

Positive WB detected inA549 cells, HeLa cells, K-562 cells, LNCaP cells, Jurkat cells, COLO 320 cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83515-4-RR is MRPL40 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MRPL40 fusion protein Ag10341
Full Name mitochondrial ribosomal protein L40
Calculated Molecular Weight 206 aa, 24 kDa
Observed Molecular Weight18-24 kDa
GenBank Accession NumberBC009707
Gene Symbol MRPL40
Gene ID (NCBI) 64976
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MRPL40, also known as NLVCF and URIM, belongs to the mitochondrion-specific ribosomal protein mL40 family. MRPL40 encodes the mitochondrial ribosomal protein L40 for the larger 39S subunit of mitochondrial ribosomes. MRPL40 is widely expressed in human organs and tissues, including the heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, ovary, small intestine, colon, peripheral blood lymphocyte (PBL), and testis. MRPL40 is also expressed in human fetuses and mouse embryos, suggesting its important role in fetal development (PMID: 36891938).

Protocols

Product Specific Protocols
WB protocol for MRPL40 antibody 83515-4-RRDownload protocol
IHC protocol for MRPL40 antibody 83515-4-RRDownload protocol
IF protocol for MRPL40 antibody 83515-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols