CoraLite® Plus 555-conjugated MRPL12 Polyclonal antibody

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  IF Staining of HepG2 using CL555-14795

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 555 MRPL12 antibody (CL555-14795) at dilution of 1:200, CL488-phalloidin (green).

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  FC experiment of Hela using CL555-14795

  1X10^6 Hela cells were intracellularly stained with 0.8 ug CoraLite® Plus 555 Anti-Human MRPL12 (CL555-14795) (red), or 0.8 ug CoraLite®555-conjugated Rabbit IgG control Rabbit PolyAb (CL555-30000, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• Featured Product
• KD/KO Validated

MRPL12 Polyclonal Antibody for IF/ICC, FC (Intra)

Cat No. CL555-14795

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Synonyms

5c5-2, Large ribosomal subunit protein bL12m, MRP-L12, MRPL7, MRPL7/L12

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: Hela cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL555-14795 targets MRPL12 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MRPL12 fusion protein Ag6504
• Full Name: mitochondrial ribosomal protein L12
• Calculated Molecular Weight: 21 kDa
• Observed Molecular Weight: 21 kDa
• GenBank Accession Number: BC002344
• Gene Symbol: MRPL12
• Gene ID (NCBI): 6182
• RRID: AB_2934640
• Conjugate: CoraLite® Plus 555 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 554 nm / 570 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P52815
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Mammalian MRPL12 was the first mitochondrial ribosomal protein to be characterized, and is encoded by a nuclear gene. The protein forms a homodimer, localizes predominantly to mitochondria and binds to the large mitochondrial ribosomal subunit [PMID:862670]. It is required for CycD/Cdk4-induced cell growth. In addition, forming a ribosomal stalk with MRPL7, MRPL12 is required for the recruitment of translation elongation factors Tu and G to the large ribosomal subunit [PMID:12370014]. It also a part of a novel regulatory mechanism that coordinates mitochondrial transcription with translation and/or ribosome biogenesis during human mitochondrial gene expression. The antibody reacts specifically with 21kDa MRPL12 protein.