MRPL12 Polyclonal antibody

• Featured Product
• KD/KO Validated

MRPL12 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

32

Cat no : 14795-1-AP

Synonyms

RPML12, MRPL7/L12, MRPL7, MRP-L12, Large ribosomal subunit protein bL12m

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Validation Data Gallery

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  WB analysis using 14795-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14795-1-AP (MRPL12 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14795-1-AP

  WB result of MRPL12 antibody (14795-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MRPL12 transfected HeLa cells.

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  IP experiment of HeLa using 14795-1-AP

  IP result of anti-MRPL12 (IP:14795-1-AP, 4ug; Detection:14795-1-AP 1:500) with HeLa cells lysate 2000ug.

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  IHC staining of human colon cancer using 14795-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14795-1-AP (MRPL12 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 14795-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14795-1-AP (MRPL12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 14795-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MRPL12 antibody (14795-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of HepG2 using 14795-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MRPL12 antibody (14795-1-AP) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of HepG2 using 14795-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MRPL12 antibody (14795-1-AP) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  FC experiment of HeLa using 14795-1-AP

  1x10^6 HeLa cells were intracellularly stained with 0.4 ug MRPL12 Polyclonal antibody (14795-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: COLO 320 cells, HeLa cells, MCF-7 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 30 publications below
• IHC: See 3 publications below
• IF: See 5 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Product Information

14795-1-AP targets MRPL12 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MRPL12 fusion protein Ag6504
• Full Name: mitochondrial ribosomal protein L12
• Calculated Molecular Weight: 21 kDa
• Observed Molecular Weight: 21 kDa
• GenBank Accession Number: BC002344
• Gene Symbol: MRPL12
• Gene ID (NCBI): 6182
• RRID: AB_2250805
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Mammalian MRPL12 was the first mitochondrial ribosomal protein to be characterized, and is encoded by a nuclear gene. The protein forms a homodimer, localizes predominantly to mitochondria and binds to the large mitochondrial ribosomal subunit [PMID:862670]. It is required for CycD/Cdk4-induced cell growth. In addition, forming a ribosomal stalk with MRPL7, MRPL12 is required for the recruitment of translation elongation factors Tu and G to the large ribosomal subunit [PMID:12370014]. It also a part of a novel regulatory mechanism that coordinates mitochondrial transcription with translation and/or ribosome biogenesis during human mitochondrial gene expression. The antibody reacts specifically with 21kDa MRPL12 protein.

Publications

Species	Application	Title
human	WB	Cell; An in vitro system to silence mitochondrial gene expression.; Authors - Luis Daniel Cruz-Zaragoza
human	WB	Mol Cell; Identification of TMEM126A as OXA1L-interacting protein reveals cotranslational quality control in mitochondria; Authors - Sabine Poerschke
	WB	Mol Cell; A kinetic dichotomy between mitochondrial and nuclear gene expression processes; Authors - Erik McShane
human	WB	Nucleic Acids Res; The human Obg protein GTPBP10 is involved in mitoribosomal biogenesis.; Authors - Elena Lavdovskaia
human	WB	Nucleic Acids Res; METTL15 introduces N4-methylcytidine into human mitochondrial 12S rRNA and is required for mitoribosome biogenesis.; Authors - Lindsey Van Haute
human	WB	Genome Biol; Balanced mitochondrial and cytosolic translatomes underlie the biogenesis of human respiratory complexes; Authors - Iliana Soto