MLLT1 Recombinant antibody

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  WB analysis using 83242-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83242-3-RR (MLLT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 83242-3-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MLLT1 antibody (83242-3-RR, Clone: 230519E7 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  FC experiment of HeLa using 83242-3-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug MLLT1 Recombinant antibody (83242-3-RR, Clone:230519E7) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  Affinity and Kinetic Characterization of 83242-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83242-3-RR against Human MLLT1 were performed. The affinity constant is below 1 pM.

MLLT1 Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 83242-3-RR

Clone No.230519E7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

YEATS1, Protein ENL, MLLT 1, LTG19, ENL

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Tested Applications

• Positive WB detected in: Jurkat cells, Raji cells, HeLa cells, K-562 cells
• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83242-3-RR targets MLLT1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: MLLT1 fusion protein Ag34210
• Full Name: myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 1
• Calculated Molecular Weight: 62 kDa
• Observed Molecular Weight: 75 kDa
• GenBank Accession Number: BC148806
• Gene Symbol: MLLT1
• Gene ID (NCBI): 4298
• RRID: AB_3670918
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q03111
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.