CoraLite® Plus 488-conjugated MLKL Monoclonal antibody

• Featured Product
• KD/KO Validated

MLKL Monoclonal Antibody for IF/ICC, IF-P

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

IF/ICC, IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Publications

2

CloneNo.

3D4C6

Cat no : CL488-66675

Synonyms

Mixed lineage kinase domain-like protein, hMLKL, 3D4C6

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Validation Data Gallery

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  IF Staining of human liver cancer using CL488-66675

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite® Plus 488-conjugated MLKL antibody (CL488-66675, Clone: 3D4C6 ) at dilution of 1:100.

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  IF Staining of human liver cancer using CL488-66675

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CL488-66675 (MLKL antibody) at dilution of 1:50.

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  IF Staining of HepG2 using CL488-66675

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 MLKL antibody (CL488-66675, Clone: 3D4C6 ) at dilution of 1:200.

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Tested Applications

• Positive IF-P detected in: human liver cancer tissue
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 2 publications below
• FC: See 1 publications below

Product Information

CL488-66675 targets MLKL in IF/ICC, IF-P applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: MLKL fusion protein Ag15188
• Full Name: mixed lineage kinase domain-like
• Calculated Molecular Weight: 471 aa, 54 kDa
• Observed Molecular Weight: 54 kDa
• GenBank Accession Number: BC028141
• Gene Symbol: MLKL
• Gene ID (NCBI): 197259
• RRID: AB_2883366
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Mixed lineage kinase domain like pseudokinase (MLKL), belongs to the protein kinase superfamily, has two MW of 54 and 30 kDa. MLKL plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in necroptosis pathway. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children.

Publications

Species	Application	Title
mouse	IF,FC	J Transl Med; TREM-1 triggers necroptosis of macrophages through mTOR-dependent mitochondrial fission during acute lung injury; Authors - Wen-Jing Zhong
	IF	Int J Biol Sci; Neutrophil extracellular traps trigger alveolar epithelial cell necroptosis through the cGAS-STING pathway during acute lung injury in mice; Authors - Han-Xi Sha