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  • Featured Product
  • KD/KO Validated

MLH1 Monoclonal antibody, PBS Only

MLH1 Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, rat

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2F12C4

Cat no : 67350-1-PBS

Synonyms

COCA2, FCC2, hMLH1, HNPCC, HNPCC2, MLH1, MutL protein homolog 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MLH1 Monoclonal antibody (67350-1-Ig)

    WB analysis using 67350-1-Ig (same clone as 67350-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67350-1-Ig (MLH1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67350-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using MLH1 Monoclonal antibody (67350-1-Ig)

    WB analysis of HeLa using 67350-1-Ig (same clone as 67350-1-PBS)

    WB result of MLH1 antibody (67350-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MLH1 transfected HeLa cells. This data was developed using the same antibody clone with 67350-1-PBS in a different storage buffer formulation.

Product Information

67350-1-PBS targets MLH1 in WB, Indirect ELISA applications and shows reactivity with Human, rat samples.

Tested Reactivity Human, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen MLH1 fusion protein Ag27723
Full Name mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli)
Calculated Molecular Weight 756 aa, 85 kDa
Observed Molecular Weight 85-100 kDa, 40-45 kDa
GenBank Accession NumberBC006850
Gene Symbol MLH1
Gene ID (NCBI) 4292
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

MLH1, also named as COCA2, belongs to the DNA mismatch repair mutL/hexB family. It heterodimerizes with PMS2 to form MutL alpha which is a component of the post-replicative DNA mismatch repair system (MMR). MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. MLH1 also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. MLH1 heterodimerizes with MLH3 to form MutL gamma which plays a role in meiosis.(PMID: 16873062, PMID: 18206974) Defects in MLH1 are the cause of hereditary non-polyposis colorectal cancer type 2 (HNPCC2). Defects in MLH1 are a cause of mismatch repair cancer syndrome (MMRCS). Defects in MLH1 are a cause of Muir-Torre syndrome (MTS). Defects in MLH1 are a cause of susceptibility to endometrial cancer. Western blot analysis with an MLH1 antibody detected a 85-100 kDa band. Full-length human MLH1 is specifically cleaved into degradation products of 40-45 kDa by caspase-3 (PMID: 15087450, PMID: 19603033). This antibody is specific to MLH1.