MLH1 Monoclonal antibody

• Featured Product
• KD/KO Validated

MLH1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2F12C4

Cat no : 67350-1-Ig

Synonyms

COCA2, FCC2, hMLH1, HNPCC, HNPCC2, MLH1, MutL protein homolog 1

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Validation Data Gallery

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  WB analysis using 67350-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67350-1-Ig (MLH1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 67350-1-Ig

  WB result of MLH1 antibody (67350-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MLH1 transfected HeLa cells.

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Tested Applications

• Positive WB detected in: A431 cells, HeLa cells, Caco-2 cells, COLO 320 cells, HEK-293 cells, Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67350-1-Ig targets MLH1 in WB, ELISA applications and shows reactivity with Human, rat samples.

• Tested Reactivity: Human, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: MLH1 fusion protein Ag27723
• Full Name: mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli)
• Calculated Molecular Weight: 756 aa, 85 kDa
• Observed Molecular Weight: 85-100 kDa, 40-45 kDa
• GenBank Accession Number: BC006850
• Gene Symbol: MLH1
• Gene ID (NCBI): 4292
• RRID: AB_2918485
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MLH1, also named as COCA2, belongs to the DNA mismatch repair mutL/hexB family. It heterodimerizes with PMS2 to form MutL alpha which is a component of the post-replicative DNA mismatch repair system (MMR). MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. MLH1 also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. MLH1 heterodimerizes with MLH3 to form MutL gamma which plays a role in meiosis.(PMID: 16873062, PMID: 18206974) Defects in MLH1 are the cause of hereditary non-polyposis colorectal cancer type 2 (HNPCC2). Defects in MLH1 are a cause of mismatch repair cancer syndrome (MMRCS). Defects in MLH1 are a cause of Muir-Torre syndrome (MTS). Defects in MLH1 are a cause of susceptibility to endometrial cancer. Western blot analysis with an MLH1 antibody detected a 85-100 kDa band. Full-length human MLH1 is specifically cleaved into degradation products of 40-45 kDa by caspase-3 (PMID: 15087450, PMID: 19603033). This antibody is specific to MLH1.