Mouse MIF Recombinant antibody, PBS Only (Capture)

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  WB analysis using 83199-2-RR (same clone as 83199-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83199-2-RR (MIF antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83199-2-PBS in a different storage buffer formulation.

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  IF Staining of NIH/3T3 using 83199-2-RR (same clone as 83199-2-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using MIF antibody (83199-2-RR, Clone: 230529F2 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 83199-2-PBS in a different storage buffer formulation.

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  FC experiment of RAW 264.7 using 83199-2-RR (same clone as 83199-2-PBS)

  1x10^6 RAW 264.7 cells were intracellularly stained with 0.25 ug MIF Recombinant antibody (83199-2-RR, Clone:230529F2) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83199-2-PBS in a different storage buffer formulation.

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  FC experiment of Neuro-2a using 83199-2-RR (same clone as 83199-2-PBS)

  1x10^6 Neuro-2a cells were intracellularly stained with 0.25 ug MIF Recombinant antibody (83199-2-RR, Clone:230529F2) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83199-2-PBS in a different storage buffer formulation.

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  FC experiment of NIH/3T3 using 83199-2-RR (same clone as 83199-2-PBS)

  1x10^6 NIH/3T3 cells were intracellularly stained with 0.2 ug MIF Recombinant antibody (83199-2-RR, Clone:230529F2) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.2 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83199-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00282-1

  Cytometric bead array standard curve of MP00282-1, Mouse MIF Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83199-2-PBS. Detection antibody: 83199-1-PBS. Standard: Eg0574. Range: 3.13-100 ng/mL.

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  Affinity and Kinetic Characterization of 83199-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83199-2-RR against Mouse MIF were performed. The affinity constant is 0.131 nM.

MIF {ptg:Brand} Recombinant Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Cat No. 83199-2-PBS

Clone No.230529F2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Synonyms

GIF, 230529F2

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Product Information

83199-2-PBS targets MIF as part of a matched antibody pair:

MP00282-1: 83199-2-PBS capture and 83199-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: macrophage migration inhibitory factor
• Calculated Molecular Weight: 13KD
• Observed Molecular Weight: 13 kDa
• GenBank Accession Number: NM_010798.2
• Gene Symbol: Mif
• Gene ID (NCBI): 17319
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P34884
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

Macrophage migration inhibitory factor (MIF) is a homo-trimeric protein that acts as a pleiotropic pro-inflammatory cytokine. It is involved in various functions, including leukocyte recruitment, inflammation, immune responses, cell proliferation, tumorigenesis, and counter-regulation of glucocorticoids (GC). MIF is a highly conserved protein of 12.5 kDa, with evolutionarily ancient homologues in plants, protozoans, nematodes, and invertebrates.