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MED21 Recombinant antibody

MED21 Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83510-1-RR
Clone No.240419E2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

SURB7, SRB7, hSrb7, 240419E2

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, L02 cells, HEK-293T cells
Positive FC (Intra) detected inHeLa cells, U-87 MG cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83510-1-RR targets MED21 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MED21 fusion protein Ag8568 Predict reactive species
Full Name mediator complex subunit 21
Calculated Molecular Weight 144 aa, 16 kDa
Observed Molecular Weight13-16 kDa
GenBank Accession NumberBC008380
Gene Symbol MED21
Gene ID (NCBI) 9412
RRIDAB_3671135
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13503
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MED21, also known as SRB7 and SURB7,belongs to the Mediator complex subunit 21 family. MED21 is a small essential subunit of the middle domain of the Mediator, which is conserved in all eukaryotes. MED21 is thought to play an important role in transcriptional activation and repression. MED21 regulates the transcription of nearly all RNA polymerase II-dependent genes (PMID: 16758199, PMID: 30559736).

Protocols

Product Specific Protocols
WB protocol for MED21 antibody 83510-1-RRDownload protocol
FC protocol for MED21 antibody 83510-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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