MED21 Recombinant antibody

MED21 Uni-rAb^TM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240419E2

Cat no : 83510-1-RR

Synonyms

SURB7, SRB7, hSrb7, 240419E2

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Validation Data Gallery

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  WB analysis using 83510-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83510-1-RR (MED21 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  FC experiment of HeLa using 83510-1-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug MED21 Recombinant antibody (83510-1-RR, Clone:240419E2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  FC experiment of U-87 MG using 83510-1-RR

  1x10^6 U-87 MG cells were intracellularly stained with 0.25 ug MED21 Recombinant antibody (83510-1-RR, Clone:240419E2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

• 

  Affinity and Kinetic Characterization of 83510-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83510-1-RR against Human MED21 were performed. The affinity constant is 2.06 nM.

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Tested Applications

• Positive WB detected in: HeLa cells, L02 cells, HEK-293T cells, HepG2 cells
• Positive FC (Intra) detected in: HeLa cells, U-87 MG cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83510-1-RR is MED21 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: MED21 fusion protein Ag8568
• Full Name: mediator complex subunit 21
• Calculated Molecular Weight: 144 aa, 16 kDa
• Observed Molecular Weight: 13 kDa
• GenBank Accession Number: BC008380
• Gene Symbol: MED21
• Gene ID (NCBI): 9412
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MED21, also known as SRB7 and SURB7,belongs to the Mediator complex subunit 21 family. MED21 is a small essential subunit of the middle domain of the Mediator, which is conserved in all eukaryotes. MED21 is thought to play an important role in transcriptional activation and repression. MED21 regulates the transcription of nearly all RNA polymerase II-dependent genes (PMID: 16758199, PMID: 30559736).