CoraLite® Plus 750-conjugated MDM1 Recombinant antibody

MDM1 Recombinant Antibody for WB

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB

Conjugate

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

230425E10

Cat no : CL750-83141-2

Synonyms

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Validation Data Gallery

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  WB analysis using CL750-83141-2

  Various lysates were subjected to SDS PAGE followed by western blot with CL750-83141-2 (MDM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: Raji cells, HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL750-83141-2 targets MDM1 in WB applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: MDM1 fusion protein Ag10541
• Full Name: Mdm1 nuclear protein homolog (mouse)
• Calculated Molecular Weight: 81 kDa
• Observed Molecular Weight: 102 kDa
• GenBank Accession Number: BC028355
• Gene Symbol: MDM1
• Gene ID (NCBI): 56890
• Conjugate: CoraLite® Plus 750 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 755 nm / 780 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Mdm1 was upregulated during ciliogenesis in multiciliated tracheal epithelial cells and localized to centrioles and cilia when transfected into NIH/3T3 cells. Mdm1 is a microtubule-binding protein that inhibits centrosome duplication when overexpressed. Proteomic analysis revealed that Mdm1 was also detected in the mouse photoreceptor sensory cilium complex and the outer segment (OS) of photoreceptor cells in the retina, suggesting that Mdm1 might be involved in the regulation of the functions and structure of the centrosome, as well as cilia. (PMID: 36171205)